Correlation of some risk factors with acute leukemia diagnoses by flow cytometry in Iraq

الملخص EN

This study has been carried out in Erbil-Iraq, 52 patients were diagnosis as acute leukemia with flow cytometry which they were admitted to Nanakaly Hospital for Blood Disease.

27 (52 %) acute lymphoblastic leukemia (ALL) in which CD45 and Side Scatter (SSC) explained that 92 % of the blast cells were B lymphocyte, according to cluster of differentiation (CD) and the common precursor Blineage immunological marker in B-ALL in this study was CD10 positive (77.9 %) and CD19 was (65.4 %) and CD34 was (39.5 %) respectively, while negative forCD13, CD117, cMPO and CD79 because less than 20 %.

CD45 and SSC distribution of the T-ALL cells explained that 92.1 % of the blast cells were T lymphocyte, and the common precursor T-lineage immunological marker in T-ALL was CD3 positive (80.4 %) and CD2 was (82.7 %) and CD5 was (77.6 %) respectively, while negative forCD10, CD19, CD1a cMPO, CD79, CD7, TCR ALPH, BETA and TCR GAMMA, DELTA.

And 25 (48 %) were acute myeloid leukemia (AML), in which CD45 and SSC distribution of the cells was explained that 48.5 % of the blast cells were granulocyte, and common immunological marker in AML was CD cMPO (70.4 %) of cells were positive, and CD33 (50.4 %) CD64 (34.4 %), while CD15 (26.7), CD13 (27.1), CD117 (20.3), while were negative for CD4, CD34, and CD79 because less than 20 %.

An appearance increase of blood group antigen A 26 (50 %) and O 17 (33 %) was noted among leukemia patients.

Generally occurrence of male patients was higher 29 (56 %) than female 23 (44 %).

Childs number and percentage was higher 28 (56 %) than adult 24(46%).The statistical analysis with Chi-squre indicates that blood group and age have affected significantly on the type of leukemia at the level of significant 0.05 ,but the effect of sex was non significant.