Molecular studies on Salmonella species isolated from food products of animal origin

الملخص EN

Foodstuffs of animal origin may present intrinsic hazards, due to microbiological contamination.

This study was conducted to determine Salmonella spp.in some types of animal source foods.

Bacteriological examination of total 246 raw food samples of animal origin, (raw milk, (105) ; beef meat (31) and poultry meat (110), collected from different localities in Ismailia City, showed isolation of 4 isolates of Salmonella spp., at percentage of 1.62 % of the examined samples.

Serological studies of the isolated Salmonella revealed identification of Salmonella Entritidis O9 H1g, m H2-(2 isolates) and Salmonella Kossein O2 H1l,v H21,5 (2 isolates).

The isolated Salmonella (100 %) showed sensitivity to Polymyxin B (PB) 300 units, (Polypeptides).

On the other hand, 50 % of the total tested Salmonella strains were resistant against 4 antimicrobial groups, (B-Lactams, B-Lactam / B-lactamase inhibitor combinations, Aminoglycosides and Tetracyclines), so considered multi drug resistant.

Isolated Salmonella strains were submitted to molecular studies for detection of the specific DNA bands for invA (Salmonella invasion protein), stn (enterotoxin), avrA (recombinant protein) and SopB (effector protein) gene factors by using PCR technique.