A light microscopic study on the effects of the electromagnetic waves arising from the mobile phone on the rat cerebellum

الملخص EN

This work was performed to study the effects of the elctromagnetic waves emitted from the mobile phone on the cerebellar cortex of adult albino rats.

A total number of 48 rats were used in this study.

The animals were divided into three groups, control, sham-exposed and exposed.

Mobile phones were switched on for 20 minutes daily for two months.

The animals of each group were scarificed at the end of the experiment.

Intra-cardiac perfusion with a fixative was performed.

The cerebellum of each animal was extracted and processed for examination.

For examination by light microscope, Gallocyanine stain, toluidine blue stain and Golgi impregnation technique were used.

Gallocyanine stain was performed for evaluation of the layering pattern and cellular constituents of each layer.

Toluidine blue stain was used for examination of semithin sections.

Golgi impregnation technique was limited for evaluating the cerebellar granular and Golgi neurons.

In the control group, there was a complete and mature layering pattern of the cerebellar cortex.

The cerebellar cortex consists of an outer molecular layer, middle Purkinje cell layer and an inner granular cell layer.

There were no observable changes in either the layering pattern or the cell constituents in all layers of the cerebellar cortex in the sham exposed group.In the exposed group, there was no observable changes in the layering pattern of the cerebellar cortex.

However, there were darkly stained neurons and empty spaces in the granular cell layer denoting suggestible neuronal loss.