Simultaneous determination of quaternary mixture of sulfamethoxazole, trimethoprim, propylparaben and methylparaben in pharmaceutical formulations using mean centering of ratio spectra

الملخص EN

Spectrophotometric method is proposed for the simultaneous determination of quaternary mixture of sulfamethoxazole, trimethoprim, propylparaben and methylparaben in pharmaceutical formulations without prior separation steps, using mean centering of ratio spectra.

The method is based on the difference in the absorption spectra for sulfamethoxazole, trimethoprim, propylparaben and methylparaben.

The method allows rapid and accurate determination of the four components.

The results showed that the method was capable to simultaneous determination of 2.5-64.0, 2.0-30.0, 0.16-20.0 and 0.8-20.0 μg / mL each of sulfamethoxazole, trimethoprim, propylparaben and methylparaben.

The results obtained were statistically compared with a developed classical least squares method (CLS).

No significant difference was found between the two proposed methods.

The proposed method has been successfully applied to simultaneous determination of sulfamethoxazole, trimethoprim, propylparaben and methylparaben in synthetic and pharmaceutical formulations.