Staphylococcal nuclease removes escherichia coli and klebsiella pneumoniae previously adhered to uroepithelial cells

الملخص EN

Twenty-seven S.

aureus isolates were obtained from patients referring various hospitals in Baghdad.

Only 17 isolates produced DNase.

SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar.

Nevertheless, only those phenotypically-producer of DNase harboured nuc gene.

Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column.

SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight.

A matter indicates that the enzyme is consistently pure.

Results proved that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; Escherichia coli and Klebsiella pneumoniae attached to the uroepithelial cells.