Screening, selection and production of extracellular methanol and ethanol tolerant lipase from Acinetobacter sp. K5b4
العناوين الأخرى
فحص و اختيار و إنتاج لايبيز خارج خلوي قادر على تحمل الميثانول و الإيثانول من Acinetobacter sp. K5b4
مقدم أطروحة جامعية
مشرف أطروحة جامعية
أعضاء اللجنة
al-Zarani, Wail Ali
al-Sharfa, Khalid
al-Nuaymat, Sulayman
الجامعة
جامعة مؤتة
الكلية
كلية العلوم
القسم الأكاديمي
قسم الأحياء
دولة الجامعة
الأردن
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2014
الملخص الإنجليزي
-A total of 34 of bacterial isolates with extracellular lipolytic activity were isolated from hydrocarbons contaminated soil samples collected from 7 different petrol stations and car repair shops in Al-karark, south of Jordan.
Seven of these isolates were able to retain100% of their original activity after exposure to methanol and ethanol organic solvents.
Among the seven isolates and based on its relative activity, the lipase produced by the bacterial isolate K5b4 exhibited the highest stability in presence of different concentrations of methanol and ethanol in the assay solution and was selected for further studies.
The identification of the K5b4 bacterial isolate using the RapID™ ONE System and 16S rRNA gene sequencing were close in identity to Acinetobacter sp.
The optimization of the physiochemical parameters of the growth conditions resulted in maximum lipase production of 31.5 mU/mL when the optimized culture media (pH 7.0) inoculated with 0.4% (v/v) inoculums size and incubated at 30 oC and 150 rpm agitation speed.
The optimum chemical composition of the growth medium supported the highest enzyme production was 1.0% (w/v) olive oil, 0.2% (w/v) glycerol and 0.15% (w/v) yeast extract.
Inclusion of different mineral salts in the culture media were did not enhance the enzyme production as it compared to the control experiment.
Meanwhile, the enzyme and biomass production was increased with the increase in NaCl concentration with the maximum enzyme and biomass production obtained when 0.05% (w/v) of NaCl was added to the growth media.
The inhibition of lipase production from Acinetobacter sp.
K5b4 in presence of glucose suggesting that lipase gene expression is prone to catabolic repression.
التخصصات الرئيسية
الموضوعات
عدد الصفحات
59
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Review of literature.
Chapter Three : Design and methodology.
Chapter Four : Findings and discussion and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Ananizah, Maysa Ratib. (2014). Screening, selection and production of extracellular methanol and ethanol tolerant lipase from Acinetobacter sp. K5b4. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561685
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Ananizah, Maysa Ratib. Screening, selection and production of extracellular methanol and ethanol tolerant lipase from Acinetobacter sp. K5b4. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-561685
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Ananizah, Maysa Ratib. (2014). Screening, selection and production of extracellular methanol and ethanol tolerant lipase from Acinetobacter sp. K5b4. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561685
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-561685
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر