HB A2 Quantitation by microcolumn-precise normal range and invalidity on the quantitation in presence of HBS

الملخص EN

-The reference range for Haemoglobin A2 (HbA2)by the microcolumn technique is not well defined.

Various text-books and inserts give different reference ranges.

This leads to confusion in interpretation.

Normal Saudi individuals were checked by the method, excluding all diseaseا'» them that can affect HbA2 level.

The reference range obtained was 2.

0-2.

7 (mean + SD).

This new range will decrease re-markably the overlap between normal, alpha thalassemia trait and beta thalassemia trait individuals.

Also, and in spite ofthe claim by the manufacturer to the contrary, it was shown that presence of sickle haemo-globin, places the assay ofHbA2 significantly outside the normal range, using a column that is supposed to be just for the purpose of accurate assay ofHbA2 in presence of HbS.

The range in sickle trait patients was shown to be 2.

5-3.

4 (mean + SD).