Calcium permeation and blocking effect in human leukaemia cells
المؤلفون المشاركون
Said M. V.
Faruq A. D.
Uwaydah, M. K.
المصدر
Sudan Medical Laboratory Journal
العدد
المجلد 1، العدد 1 (30 يونيو/حزيران 2011)
الناشر
جامعة أم درمان الإسلامية كلية المختبرات الطبية
تاريخ النشر
2011-06-30
دولة النشر
السودان
التخصصات الرئيسية
الموضوعات
الملخص EN
Cell-attached and inside-out patch-clamp methods were employed to identify and characterize mechanosensitive (MS) ionic channels in the plasma membrane of human myeloid leukaemia K562 cells.
A reversible activation of gadolinium-blockable mechanogated currents in response to negative pressure application was found in 58 % of stable patches (n = 317).
I-V relationships measured with a sodium-containing pipette solution showed slight inward rectification.
Data analysis revealed the presence of two different populations of channels that were distinguishable by their conductance properties (17.
2 ± 0.
3 pS and 24.
5 ± 0.
5 pS), but were indistinguishable with regard to their selective and pharmacological properties.
Ion-substitution experiments indicated that MS channels in leukaemia cells were permeable to cations but not to anions and do not discriminate between Na+ and K +.
The channels were fully impermeable to large organic cations such as Tris+ and N-methyl-D-glucamine ions (NMDG +).
Ca2+ permeation and blockade of MS channels were examined using pipettes containing different concentrations of Ca2 +.
In the presence of 2 mM CaCl2, when other cations were impermeant, both outward and inward single-channel currents were observed ; the I-V relationship showed a unitary conductance of 7.
7 ± 1.
0 pS.
The relative permeability value, PCa / PK, was equal to 0.
75, as estimated at physiological Ca2+ concentrations.
Partial or full inhibition of inward Ca2 + currents through MS channels was observed at higher concentrations of external Ca2 + (10 or 20 mM).
No MS channels were activated when using a pipette containing 90 mM CaCl2.
Monovalent mechanogated currents Sud Med Lab J.
Vol (1) : 62-77 (2011) were not significantly affected by extracellular Ca2+ at concentrations within the physiological range (0-2 mM), and at some higher Ca2+ concentrations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Said M. V.& Faruq A. D.& Uwaydah, M. K.. 2011. Calcium permeation and blocking effect in human leukaemia cells. Sudan Medical Laboratory Journal،Vol. 1, no. 1.
https://search.emarefa.net/detail/BIM-576098
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Said M. V.…[et al.]. Calcium permeation and blocking effect in human leukaemia cells. Sudan Medical Laboratory Journal Vol. 1, no. 1 (2011).
https://search.emarefa.net/detail/BIM-576098
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Said M. V.& Faruq A. D.& Uwaydah, M. K.. Calcium permeation and blocking effect in human leukaemia cells. Sudan Medical Laboratory Journal. 2011. Vol. 1, no. 1.
https://search.emarefa.net/detail/BIM-576098
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-576098
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر