Occurrence and detection of ampc b-lactamases among enterobacteriaceae isolates from patients at Ain Shams University Hospital
المؤلفون المشاركون
Adil, Lamya A.
al-Hadi, Suha A.
المصدر
The Egyptian Journal of Medical Human Genetics
العدد
المجلد 16، العدد 3 (30 سبتمبر/أيلول 2015)، ص ص. 239-244، 6ص.
الناشر
الجمعية المصرية للأمراض الوراثية
تاريخ النشر
2015-09-30
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
AmpC b-lactamases are often plasmid mediated that hydrolyze all b-lactam antibiotics except cefepime and carbapenems.
Aim of the study: We aimed to evaluate the presence of AmpC b-lactamase among Enterobacteriaceae isolates separated from patients with nosocomial infections, and to detect the genetic basis for AmpC production in these strains.
Subjects and methods: 50 AmpC b-lactamase Enterobacteriaceae were analyzed for the presence of AmpC production.
Three phenotypic AmpC confirmation assays (AmpC E test, the disk approximation test, Amp C EDTA disc) were able to detect the majority of AmpC-positive strains correctly.
Molecular detection of plasmid mediated AmpC by multiplex PCR was conducted on them.
Results: The results show that from the 148 total isolates obtained from ICU admitted patients with suspected nosocomial infections, 50 (33.8%) were AmpC b-lactamase isolates.
For the 50 AmpC isolates, all phenotypic Amp C tests gave a positive result.
Among these isolates, plasmid encoded AmpC genes were detected by multiplex PCR in 46 (92%) isolates, which included Klebsiella pneumoniae (n=22) (47.8%), Escherichia coli (n=15) (32.6%), and Proteus mirabilis (n= 9) (19.6%).
One E.
coli, one K.
pneumoniae, and two P.
mirabilis isolates showed no AmpC gene.
The most prevalent AmpC gene was that belonging to family CMY-1 which was detected in 73.9% (34/46) of all isolates.
Conclusion: It could be concluded that: Amp C producing isolates among Enterobacteriaceae strains have been increasingly recognized in the Ain Shams University Hospital.
Thus, molecular identification of the genes encoding AmpC would be essential for a reliable epidemiological investigation of their transmission in hospitals.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Hadi, Suha A.& Adil, Lamya A.. 2015. Occurrence and detection of ampc b-lactamases among enterobacteriaceae isolates from patients at Ain Shams University Hospital. The Egyptian Journal of Medical Human Genetics،Vol. 16, no. 3, pp.239-244.
https://search.emarefa.net/detail/BIM-578260
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Hadi, Suha A.& Adil, Lamya A.. Occurrence and detection of ampc b-lactamases among enterobacteriaceae isolates from patients at Ain Shams University Hospital. The Egyptian Journal of Medical Human Genetics Vol. 16, no. 3 ( 2015), pp.239-244.
https://search.emarefa.net/detail/BIM-578260
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Hadi, Suha A.& Adil, Lamya A.. Occurrence and detection of ampc b-lactamases among enterobacteriaceae isolates from patients at Ain Shams University Hospital. The Egyptian Journal of Medical Human Genetics. 2015. Vol. 16, no. 3, pp.239-244.
https://search.emarefa.net/detail/BIM-578260
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 244
رقم السجل
BIM-578260
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر