دراسة المحتوى الفينولي لأنسجة بعض أصناف نخيل التمر Phoenix dactylifera L. و تأثيرها في تطور النسيج القمي المزروع خارج الجسم الحي

الملخص EN

This study was conducted at the tissue culture lab that belongs to the Date Palm center/ University of Basra.

The study is concerned with culturing the shoot tip of four cultivars of date palm (Hillawi, Barhi, Khadrawi, Sair ) on MS media to induce the initial callus, embryogenic callus, somatic embryos and plantlets.

The percentage of total phenols of the lateral buds of the above four mentioned cultivars was estimated as well as the processes of developing the cultured tissue.

The study showed the following: A significant increase of Khadrawi in the total phenols amount as compared to the other cultivars which was 139 Mg.g-1 fresh weight .

The sair showed the least content of phenols amount 90 Mg.g-1 fresh weight .

The lateral bud showed a significant increase in tissue content of total phenols, the amount was 273 Mg.g-1 fresh weight, whereas the initial callus showed a significant decrease as compared to the other tissues.

The total phenols in this case was 18.5 Mg.g-1 fresh weight .

The high phenols content of cultivars led to lower the percentage 0f contamination and increase browning.

They also led to decrease the induction and quantity of callus from shoot tip cultures.

The results showed a negative significant correlation between the tissue content of phenols and the percentage of contamination and induction of callus by correlation coefficient which were estimated as ( - 0.997، - 0.985 ) respectively.

There was a positive significant between the tissue content of phenols and browning by correlation coefficient which is estimated as ( 0.986 ).