Synthesis of N-(4-Chlorophenyl)‎-2-methoxy-4-methylbenzamide and spectrofluorometric study

الملخص EN

N-(4-Chlorophenyl)-2-methoxy-4-methylbenzamide (3) is synthesized from reacting carboxylic acid (1) with 4-chloroaniline (2) by using N,N’-dicyclohexylcarbodiimide (DCC) and 1-hydroxybezotriazole (HOBt) as coupling reagent.

The product is confirmed by IR, 1H-NMR and elemental analysis .

The conditions of fluorescence behaviours are studied such as solvent, pH, temperature, time, concentration effect and binding constant at λe x 340 nm and λem 380 nm.

The best fluorescence intensity is obtained at pH = 5 and temperature 25 0C, while the fluorescence intensity remains stable with increasing of time.

The LOD, LOQ, S.

D and R.

S.D % of compound 3 are found 0.2691 mg.

L-1, 0.898 mg.L-1, 0.

591 and 1.

369 respectively