Extraction of aloin from Aloe Vera plant and study its effect in micronucleus formation in acute lymphoid leukemia

الملخص EN

Aloin extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase.

Partitioning of AQs[Anthraquinones] is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in Aqueous tow-phase system [ATPS].

Aloin was partially purified by using 1-propanol [NH4] 2SO4, the use of this solvent showed high efficiency 90.

61 % compared with other solvent [2-propanol and ethanol].

The concentration of aloin detected by HPLC technique, which reached to 91.

84 % as focus turns out that there is compatibility between the sample and the standard in shape and retention time which reached [1.

462] compared with the standard [1.

465].

After the treatment of blood leukemic patient cultures, three concentrations of aloin were used [100, 200 and 400] μg/ml, the last concentration showed the ability to reduce the coefficient of micronucleus formation to [0.

0062] micronucleus / cell, compared with non- treated culture which reached to [0.

0236] micronucleus/cell.