Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of ocular toxoplasmosis
العناوين الأخرى
اختبار الأميونوبلوتنج و الأليزا لتشخيص توكسوبلازما العين
المؤلفون المشاركون
Ali, Umar Muhammad
Shahin, Maha Sayyid Ibrahim
المصدر
العدد
المجلد 25، العدد 2 (30 إبريل/نيسان 2001)، ص ص. 71-86، 16ص.
الناشر
تاريخ النشر
2001-04-30
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Ocular toxoplasmosis is an important cause of blindness and visual handicap in young people, the clinical diagnosis can be suggestive but not conclusive.
Therefore.
It must be supplemented with laboratory serological tests that might, support or exclude this diagnosis.
In the present work diagnosis of ocular toxoplasmosis was based on the clinical picture and the serological, lest results of enzyme linked-immunosorbent assay (IGM-ELISA) & (IgG- ELISA) and Western blotting of Toxoplasma antigens against sera from ocular patients in the acute and chronic phases of the disease Fourty five cases of suspected ocular toxoplasmosis and five control samples were examined clinically and serologically.
The clinical diagnosis was based on the manifestation of characteristic biomicroscopic features where a white and irregular inflammatory focus is detected frequently in association with an old scar, Fundus examination revealed 5 cases in the acute phase.
27 cases in the chronic phase and 13 atypicat cases Sera were collected to be tested serologically by ELISA and Western blot technique where it was found that the diagnosis was confirmed for 82.3% (37 out of 45 cases) by ELISA ; this rate rose to 88.9% (40 out of 45 cases) by Western blot technique addding better results.
All control sera were negative by ELISA and Western blot.
Toxoploasma gondii cyst antigen was prepared, characterized by SDS-PAGE and recognized by antibodies in serum samples from patients presenting with ocular toxoplasmosis by means of Western bolt technique.
Antigenic hands, 22, 23.
21, 25.
27, 66 and 67 KDa could be characterized as antigenic markers for acute ocular totopiasnmm and 34, 35.
36, 37.
38, 39.
40, 43.
66 and 67 Kda as antigenic markers for chronic ocular toxoplasmosis In the present work immunblotting assay with sensitivity 100% and specificity 62.5% was proved to be an excellent test for diagnosis of ocular toxoplasmosis.
The present work is the first clinico-iminunoiogical study of ocular toxoplasmosis in Assiut University Hospital and the - first work in which immunoblot is applied for diagnosis of ocular toxoplasmosis in Upper –Egypt .
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. 2001. Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of ocular toxoplasmosis. Assiut Medical Journal،Vol. 25, no. 2, pp.71-86.
https://search.emarefa.net/detail/BIM-58900
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of ocular toxoplasmosis. Assiut Medical Journal Vol. 25, no. 2 (2001), pp.71-86.
https://search.emarefa.net/detail/BIM-58900
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shahin, Maha Sayyid Ibrahim& Ali, Umar Muhammad. Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of ocular toxoplasmosis. Assiut Medical Journal. 2001. Vol. 25, no. 2, pp.71-86.
https://search.emarefa.net/detail/BIM-58900
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 84-86
رقم السجل
BIM-58900
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر