Evaluation of the concentrations of products of nitric oxide oxidation and some antioxidant vitamins in sheep naturally infested with parasites

الملخص EN

The aim of the present study was to investigate the status of nitric oxide oxidation products and some antioxidant vitamins in sheep naturally infected with parasites.

The present study was carried on 40 sheep naturally infected with parasites and 10 healthy sheep served as control, Fecal examination of naturally infected group revealed the presence of, Nematodirus.

Spp, Fasciola.

Spp and Trichostrogylidea eggs and Eiemira.

Spp oocyst.

The mean fecal egg count (FEC) in sheep infected with Trichostrongylide nematode was 5600/g and 3900/g with Eiemira.

Spp oocyst.

There were no blood parasite found in blood smear.

The concentration of nitric oxide and antioxidant vitamins (C, E, ß-Carotene and retinol) were determined spectrophotometrically by chemical kits in the serum of sheep under study.

The results revealed that nitric oxide was significantly increased in diseased sheep than in control one (9.

01 ± 5.

6 = g / ml nitrate, 2.

62 ±1.

7 nitrite and 4.

60 ± 2.

32 = g / ml nitrate, 1.

54 ±1.

4 nitrite =g / ml respectively), while vitamin E, revealed significant decrease in infested sheep (0.

61± 0.

167 =g / ml, 0.

79 ± 0.

159 = g / ml respectively) also vitamin C reveled significant decrease in parasites infested group than control group (0.

092 ± 0.

042 =g/ml and 0.

126 ± 0.

037 =g / ml) on the other hand retinol and B-carotene did not significantly change in both group.

It could be concluded that nitric oxide in blood serum of the infested sheep was higher than healthy control sheep (p < 0.

05), where vitamin E and C level in serum of the infected group were lower than the control group (p < 0.

05), while the concentration of other parameters examined were not statistically different in the two groups.