Comparison of some rapid techniques used for diagnosis of brucellosis of aborted cows compared with convential method

الملخص EN

A total of 158 specimens of uterine discharges and lochia were collected from aborted cows of different localities in Egypt.

The blood samples of the same aborted cows were also collected for serological tests.

The bacteriological method was applied for isolation of Brucella organisms from 102 uterine discharges and 56 lochia of aborted cows.

Primary inoculation was done on Brucella agar plates.

The plates were incubated in the presence of 5 % CO2 in Carbon dioxide incubator for 72-120 hrs.

The isolates were initially recognized on the basis of their cultural and morphological features and biochemical identification.

DOT-ELISA was applied on the colonies plates for detection of Brucella microorganisms in the aborted materials.

The direct fluorescent antibody test (DFAT) was applied on the specimens of uterine discharges and lochia.

Samples were coated on the slides as antigens, then Brucella specific antibodies labelled with a fluorescein conjugate were added and examined under the fluorescent microscope.

The applied serological tests in this study were Rose Bengal Test (RBT), Standard Tube Agglutination Test (SAT), Rivanol Test (RT) and Enzyme Linked Immunosorbant Assay (ELISA) test.

The rate of isolation of Brucella melitensis (B.

melitensis) from aborted cows was 7.59 % from 12 isolates ; 7.84 % from 8 uterine discharges and 7.14 % from 4 lochia by cultural bacteriological method and Dot-ELISA.

The result of DFAT were 25 (15.82 %) positive ; 16 (15.69 %) from uterine discharges and 9 (16.07 %) from lochia.

The results of serological tests on the serum samples were 64 (40.5١ %), 58 (36.71 %), 55 (34.81 %) and 66 (41.77 %) positive results for RBT, SAT, RT and ELISA respectively.

Finally, we concluded that in order to eradicate and control brucellosis, we must apply a good surveillance reliable diagnostic test.

The practical serological diagnosis must be based on screening test of high sensitivity followed by a confirmatory test as ELISA test of high specificity.

A combination of serological test with FAT is usually needed for diagnosis of Brucella organisms in aborted cases.