Molecular typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene
المؤلفون المشاركون
Faiq, Ashna Jamal
Latif, Kawthar Riyad
Aziz, Ismail Husayn
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 14، العدد 1 (30 يونيو/حزيران 2015)، ص ص. 23-28، 6ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2015-06-30
دولة النشر
العراق
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص AR
-The aim of this study was to determine the genotypic characteristics of S.
aureus isolates by using coagulase gene typing and polymerase chian reaction-restriction fragment length polymorphism (PCR-RFLP) of coagulase gene.
One hundred and seventy two wound swab specimens were collected from patients in AL-Kindy Teaching Hospital, Al-Wasity Hospital, Baghdad Al-Taalemey Hospital and Al Shaheed Gazi Hospital in Baghdad during the period from September 2013 to January 2014.
All isolates were diagnosed depending on microscopical and biochemical tests.
It was found that 47(27.33%) isolates were identified as S.aureus.
The amplification of the coa gene of S.aureus strains generated 5 different genotypes: I, II, III, IV, V based on different size of polymorphism raning from 500-900bp.
The majority (44.7%) of the strains were of coa gene type II.
PCR-RFLP of coa gene exhibited 11patterns which were obtained with AluI digests of PCR products.
The number of fragments varied from one to three with sizes of the fragments varied from 80 to 700 bp.
Type IX was the most common and accounted for 27.7% of the isolates.
PCR-RFLP analysis, showed discriminatory power, reproducibility, easy interpretation and can be considered as a promising alternative for the epidemiological typing of S.aureus isolates
الملخص EN
The aim of this study was to determine the genotypic characteristics of S.
aureus isolates by using coagulase gene typing and polymerase chian reaction-restriction fragment length polymorphism (PCR-RFLP) of coagulase gene.
One hundred and seventy two wound swab specimens were collected from patients in AL-Kindy Teaching Hospital, Al-Wasity Hospital, Baghdad Al-Taalemey Hospital and Al Shaheed Gazi Hospital in Baghdad during the period from September 2013 to January 2014.
All isolates were diagnosed depending on microscopical and biochemical tests.
It was found that 47(27.33 %) isolates were identified as S.aureus.
The amplification of the coa gene of S.aureus strains generated 5 different genotypes: I, II, III, IV, V based on different size of polymorphism raning from 500-900bp.
The majority (44.7 %) of the strains were of coa gene type II.
PCR-RFLP of coa gene exhibited 11patterns which were obtained with AluI digests of PCR products.
The number of fragments varied from one to three with sizes of the fragments varied from 80 to 700 bp.
Type IX was the most common and accounted for 27.7% of the isolates.
PCR-RFLP analysis, showed discriminatory power, reproducibility, easy interpretation and can be considered as a promising alternative for the epidemiological typing of S.aureus isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Latif, Kawthar Riyad& Aziz, Ismail Husayn& Faiq, Ashna Jamal. 2015. Molecular typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Iraqi Journal of Biotechnology،Vol. 14, no. 1, pp.23-28.
https://search.emarefa.net/detail/BIM-596036
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Latif, Kawthar Riyad…[et al.]. Molecular typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Iraqi Journal of Biotechnology Vol. 14, no. 1 (2015), pp.23-28.
https://search.emarefa.net/detail/BIM-596036
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Latif, Kawthar Riyad& Aziz, Ismail Husayn& Faiq, Ashna Jamal. Molecular typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 1, pp.23-28.
https://search.emarefa.net/detail/BIM-596036
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 27-28
رقم السجل
BIM-596036
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر