Seroepidemiological and molecular study of hepatitis B virus in AL-Rusafa sector of Baghdad

العناوين الأخرى

دراسة مصلية وبائية و جزئية لفيروس التهاب الكبد نمط (ب)‎ في قاطع الرصافة لمدينة بغداد

مقدم أطروحة جامعية

al-Hammiary, Thair Kazim Fayyd

مشرف أطروحة جامعية

al-Hassani, Hayfa Hadi
Umar, Ali R.

أعضاء اللجنة

Ibrahim, Salih Jalil
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
al-Suhayl, Raghad Ghalib

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

This study attempted to determine the prevalence of HBV infection and other epidemiologic criteria among normal populations and healthcare workers (HCWs) as a risk group chosen from various hospitals and health centers.

Moreover, HBV markers among different types of this infection were studied and HBV DNA detection among individuals with HBsAg negative and anti-HBc total in Al-Rusafa sector of Baghdad.

A total of 1062 serum specimens (588 specimen from normal population, 209 specimen from HCWs, 165 specimen from HBV patients and 100 specimen from HBsAg negative blood donors) were collected and tested for detection of serological markers using ELISA technique and positive specimens for HBsAg were confirmed by VIDAS technique at the Central Public Health Laboratory.

Regarding normal population group, the seroprevalence rate of HBsAg, anti-HBs and anti-HBc Total were 1.02 %, 10.54 % and 5.44 %, respectively.

The HBsAg rate was significantly lower (P ≤ 0.05) than previous studies were done in Iraq.

Additionally, significant correlation (P ≤ 2 0.05) in seroprevalence of HBsAg was found between children below 15 years old and adult ages.

Moreover males had a higher positive HBsAg marker than females with significant importance (P ≤ 0.05) (male: female ratio 1.6:1).

Regarding seroprevalence of anti-HBs, a non-significant correlation (P ≤ 0.05) between children below 15 years old and adult ages was seen; in addition, significant correlation (P ≤ 0.05) was recorded between both sexes.

Whereas highly significant importance (P ≤ 0.01) was recorded between children below 15 years old and adult ages regarding seroprevalence of anti-HBc total as well as non-significant (P ≤ 0.05) between both sexes were noticed.

The seroprevalence of HBsAg, anti-HBs and anti-HBc total among HWCs was 0.96 %, 26.7 % and 1.44 % respectively.

The prevalence of HBsAg was significantly less than previous studies with significant correlation (P ≤ 0.05) in comparison with normal population group.

The seroprevalence rate of anti-HBs was significantly increased (P ≤ 0.05) as compared with normal population group, whereas seroprevalence of anti-HBc total was higher than that observed in normal population with a highly significant importance (P ≤ 0.01).

Regarding age groups, seroprevalence of HBsAg among HCWs was significantly increased in older ages, while no such variation was observed between males and females.

The occupation concern revealed a high seroprevalence of HBsAg among those with poor health education and experience.

The prevalence of anti-HBs among HCWs was significantly (P ≤ 0.05) increased with the advancing ages, whereas a non-significant prevalence in 3 anti-HBs (P > 0.05) was shown between both sexes.

On the other hand, a significant (P ≤ 0.05) prevalence of anti-HBs among different types of occupation was noticed in this group.

Low coverage rate of vaccine was recorded among HCWs with significant differences (P ≤ 0.05) among vaccinated, incomplete vaccinated and non- vaccinated groups.

Additionally, HCWs who had only 1st, only 2nd and those who had 3rd dose showed significant correlation (P ≤ 0.05) between number of vaccinated individuals and seroprevalence of anti-HBs and the results indicated the high effectiveness of vaccination related doses.

Moreover, HBV markers among patients with acute infection showed a high seroprevalence of anti-HBcIgM and anti-HBcTotal markers.

High rate of HBeAg with low rate of anti-HBe; in addition, absence of anti-HBs and high rate of absence of HBeAg together with anti-HBe was seen.

In chronic patient group, low rate of anti-HBcIgM, high rate of anti-HBc total, HBeAg and anti-HBe was recorded with low rates, while absence of the above markers were noticed, whereas HBsAg with anti-HBs together were present in low rate among this group.

In apparently healthy carrier group, anti-HBc IgM was absent; high rate of anti-HBc total was noticed; low rate of HBeAg, high rate of anti-HBe, low rate of absence of HBeAg and anti-HBe together and absence of anti-HBs among this group were revealed.

Comparison between markers among the three types of infection showed significant importance (P ≤ 0.05) among markers with the exception of HBeAg markers but without a significant importance.

The biochemical tests of TSB and ALT showed significant correlation (P ≤ 0.05) among the three types of infection.

Regard to the risk factors of transmission, a frequent parenteral drug 4 administration, previous blood transfusion and more than one risk factor showed significant importance (P ≤ 0.05).

Co-infection of both HDV and HCV among HBV patients existed with a low significant rate among patients with chronic infection.

High seroprevalence of HBV DNA among HBsAg negative and anti- HBcTotal positivity blood donors with significant correlation (P ≤ 0.05) between routine serological methods (ELISA) and PCR technique were indicated.

التخصصات الرئيسية

الطب البشري

الموضوعات

• علم الأوبئة
• الأمراض
• العراق
• المتبرعون بالدم
• الرصافة

عدد الصفحات

111

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-597165