Production and characterization of bioemulsifier from locally isolated Serratia marcescens S10
العناوين الأخرى
إنتاج و توصيف المستحلبات الحياتية من بكتريا serratia marcescens S10 المعزولة محليا
مقدم أطروحة جامعية
al-Mulla, Intiṣar Firawn Ahmad
مشرف أطروحة جامعية
أعضاء اللجنة
Abd al-Jalil, Sana Burhan
Abd al-Karim, Muntaha
Haydar, Nahdim Hasan
الجامعة
جامعة بغداد
الكلية
كلية العلوم
القسم الأكاديمي
قسم علوم الحياة
دولة الجامعة
العراق
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2010
الملخص الإنجليزي
Fourteen bacterial isolates belong to Serratia were isolated from different sources.
All these (14) isolates were identified according to morphological and biochemical characteristics, and confirmed by API 20E system.
The isolates were screened for their ability to produce bioemulsifier depending on the surface tension measurement (mN/m), E24% and other tests for cell-free broth after cultivation in mineral salts medium supplemented with olive oil as a sole carbon source.
Serratia marcescens (S10) isolated from insects was the best bioemulsifier producers.
The optimum conditions for bioemulsifier production from S.
marcescens (S10) were determined: mineral salts medium containing sesame oil was the best as a carbon source, ammonium sulphate was used as nitrogen source with pH (8) at (37)Cº for (9) days.
The bioemulsuifier was extracted by organic solvent (chloroformmethanol and diethylether) and the components of bioemulsifier were detected by TLC (Thin layer chromatography) and HPLC (High performance liquid chromatography) analysis which demonstrated that the bioemulsifier is containing lipid, 17.62% carbohydrate and 2.97% protein.
The bioemulsifier characterization was determined; the results showed that the bioemulsifier is active in a wide range of pHs, thermostable at (70Cº) and active in different salt concentrations (3% to 20%).
The antimicrobial activity (antibacterial and antifungal) of bioemulsifier was applied against some microorganisms, the bioemulsifier reduced the growth of bacteria (E.coli , Lesteria , Salmonella , Klebsiella and Staphylococcus aureus ) and inhibited the growth of fungi (Candida albicans, Aspergillus niger and Geotricum).
II List of contents Title Page No.
Summary
التخصصات الرئيسية
عدد الصفحات
136
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and Ddscussions.
Conclusions and recommendations
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Mulla, Intiṣar Firawn Ahmad. (2010). Production and characterization of bioemulsifier from locally isolated Serratia marcescens S10. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597322
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Mulla, Intiṣar Firawn Ahmad. Production and characterization of bioemulsifier from locally isolated Serratia marcescens S10. (Master's theses Theses and Dissertations Master). University of Baghdad. (2010).
https://search.emarefa.net/detail/BIM-597322
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Mulla, Intiṣar Firawn Ahmad. (2010). Production and characterization of bioemulsifier from locally isolated Serratia marcescens S10. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597322
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-597322
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
