Role of Proteus mirabilis DNA in comparison to candida albicans DNA in rats’ joints infection

العناوين الأخرى

دور دنا بكتريا Proteus mirabilis مقارنه بدنا الخميرة Candida albicans في إصابة مفاصل الجرذان

مقدم أطروحة جامعية

al-Khatib, Ahmad Fadil Kazim

مشرف أطروحة جامعية

al-Mathkhuri, Harith Jabbar Fahd
Ubayd, Hind Husayn

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

Septic arthritis is a key consideration in adults presenting with acute mono articular arthritis.

The incidence of septic arthritis ranges widely, between four and 29 cases per 100000 person-years, depends on population variables and preexisting structural joint abnormalities (Mathews et al., 2010).

Fourteen (11.6%) Proteus isolates were obtained from 120 specimens collected from midstream urine of patients of both sex and different ages attending deferent hospitals in Baghdad.

Identification was achieved depending on cultural characteristics, microscopic examination and biochemical tests.

The results of identification were confirmed via Vitek 2 system.

Four Candida albicans isolates were identified by microscopic examination, germ tube formation and API Candida system.

Among Proteus isolates, P.

mirabilis formed 85.7% (12 isolates) of total Proteus isolates, while P.

vulgaris appeared only in 14.3% (2 isolates) of total Proteus isolates.

Susceptibility test was done for all P.

mirabilis isolates against seven antibiotics widely used for the treatment of Proteus infections; included Amikacin, Tobromycin, Imipenem, Ciprofloxacin, Ceftazidime, Gentamicin and Trimethoprim-Sulfamethoxazole.

All Proteus mirabilis isolated from UTI developed multidrug resistance and variable degree of resistance toward Trimethoprim-Sulfamethoxazole (100%), Ciprofloxacin (66.6%), Ceftazidime (58%), Gentamicin (58%), Imipenem (25%), Tobromycin (16.6%) and Amikacin (16.6%).

DNA was isolated from P.

mirabilis isolate which developed the highest sensitivity towards the tested antibiotics.

P.

mirabilis DNA was isolated using a Wizard genomic DNA purification kit method.

Whereas, C.

albicans DNA was isolated using EZ-10 Spin column fungal genomic DNA Mini-Preps Kit.

Genomic DNA was further analyzed quantitatively and qualitatively by gel electrophoresis, which showed one band of DNA.

The absorbencies of P.

mirabilis DNA at 260 nm and 280 nm were 0.323and 0.182, respectively.

While the absorbance of C.

albicans DNA at 260 nm and 280 nm were 0.361 and 0.211, respectively.

Accordingly, the concentration of P.

mirabilis DNA was 161.5 μg/μl and the purity (OD260/280 ratio) was 1.77.

While the concentration of C.

albicans DNA was 180.5μg/μl and the purity was 1.71.

As a consequence, the extracted DNA was considered pure since that it was within the expected range of 1.7 to 2 (Deng et al., 2001).

The histological changes caused by purified DNA from P.

mirabilis and C.

albicans was investigated using rat model.

Histopathological examination of knee joints from rat intraarticulary treated with 30 μg/100 μl of P.

mirabilis DNA revealed congestion, inflammatory cells infiltration, oedema, hemorrhagic exudates due to the lysis of blood vessels and necrotic cells.

Furthermore, articular joints damage has been noticed with articular tissue in ligament and lining epithelium.

Moreover, an aggregation of lymphocyte was observed, while 30 μg/100 μl DNA of C.

albicans caused congestion and a mild infiltration of inflammatory cells with oedema and augmented infiltration reaction inside the joint space.

Serum IL-6 levels peaked (159.33 pg/ml) within 24 hours after injection of P.

mirabilis DNA intraarticularly; which was significantly higher (P< 0.01) than its level in animals injected with C.

albicans DNA (64.66 pg/ml) and TE Buffer (51 pg/ml).

After 14 days of intraarticularly injection with 30μg/100 μl of P.

mirabilis DNA, 30 μg/100 μl of C.

albicans DNA and TE buffer.

Anti-ds Ab concentration was significantly higher (P< 0.01) in P.

mirabilis group

التخصصات الرئيسية

الأحياء

عدد الصفحات

144

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-597830