Antagonistic activity evaluation against some pathogenic bacteria of fixed oils extracted from different plant seeds

العناوين الأخرى

تقيم الفعالية المضادة لبعض أنواع البكتريا الممرضة الناتجة من الزيوت الثابتة المستخلصة من بذور بعض نباتات مختلفة

مقدم أطروحة جامعية

al-Qurayshi, Raniya Sabri Hasan

مشرف أطروحة جامعية

al-Mathkhuri, Harith Jabbar Fahd
al-Shamma, Layth Muhammad Jawad

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

This study was conducted in Bacteriology laboratories in the province of Wasit during the period from February 2011 to February 2012 to determine the impact of some plant oils in growth inhibition of several pathogenic bacteria isolated from 110 specimens (urine , skin, nose and vaginal swab) of both sexes aged between (2-70 yrs) attending Al Zahra Hospital and Al Karama Hospital in Kut city.

Seventy specimens resulted positive culture.

Six bacterial species were identified; E.coli and Staphylococcus arueus (35.7% for each of them), Klebsellia pneumaniae, Pseudomonas aeruginosa, Staphylococcus epidermidis and Streptococcus pyogenes, (7.1% for each of them).

All S.

aureus were methicillin sensitive.

Oil was extracted from seeds of some plants (sunflower, rape seed, peanut, olive, safflower, sesame, cotton, soybean, castor and colocynth).

Oil percentage was calculated per plant.

Peanuts oil accounted for 40.3%, while the olive oil (envelope) had the lowest oil content (1.6%).

Superiority peanuts oil for other oils inhibiting E.

coli isolated from 15 patients suffering from female genital tract infections (vaginal) ranged in age between 25-55 years old achieving an inhibitory diameter reached 8.43 mm in concentration 100% Peanut oil is significant overlap in the inhibition of E.

coli, followed by sunflower oil diameter of 11.14 mm and 10.32 mm, respectively.

On the other hand, castor oil was the high among oils in inhibiting E.

coli isolated from 10 patients of both sexes aged between 30-70 years old presented with recurrent urinary tract infections accomplishing zone of inhibition 9.06 mm in diameter found significant overlap closely related to castor oil 100% inhibition of E.

coli diameter of 12.82 mm.

Nonetheless, S.

auereus isolated from nasal of 10 patients aged between 18-23 years of both sexes developed high susceptibility towards sesame and castor oils reaching inhibitory diameter of 12.59 and II 11.22 mm, respectively.

In concentration 100% Sesame oil is significant overlap in the inhibition of S.

aureus diameter of 17.39 mm.

However, S.

auereus isolated from 15 patients of both sexes aged 40-66 years presented with UTI showed marked susceptibility to peanut oil (10.89 mm) and significant overlap of 100% concentration of oil in the inhibition of S.

aureus14.67 mm in diameter.

S.

pyogenes isolated from burn of five patients aged 2-45 years revealed susceptibility to oils extracted from colocynth and sesame by achieving inhibitory diameter reached 9.04 and 7.32 mm in diameter, respectively.

100% concentration found significant overlap of colocynth oil in the inhibition of S.

pyogenes diameter of 11.43 mm.

As for soybean oil, which did not differ from other oils in inhibition of bacterial K.

pneumaniae isolated from burns to five patients aged 4 to 12 years.

Nevertheless, Pseudomonas aeruginosa and Staphylococcus epidermidis have not been affected by any oil under study.

The largest inhibitory diameter was accomplished by 100% concentration; whereas, control treatment did not show any inhibition to the bacterial isolates utilized in this study.

It is noteworthy to mention that well diffusion method was more accurate than disc diffusion method in all oils and bacterial isolates used in this work.

HPLC analysis revealed that the content of α-linolenic in oils of sunflower, safflower, rapeseed, soybean, olive and castor was higher than other fatty acids followed by oleic in Safflower and rape seed oils.

Also, it has been found that sesame oil contained high proportion of linoleic followed by cotton oil in its content of fatty acids but it did not affect the bacteria tested in this study compared to other oil contents.

Perhaps this is why it able to inhibit some bacterial species; which affect the cellular components in bacteria.

التخصصات الرئيسية

الأحياء

الموضوعات

• علم الأحياء الدقيقة
• البذور
• الأمراض الجرثومية
• البكتيريا

عدد الصفحات

110

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-598137