Environmental and identification study of algae present in three drinking water plants in Baghdad and molecular detection of some toxigenic cyanobacteria

العناوين الأخرى

دراسة بيئية و تشخيصية للطحالب الموجودة في ثلاث محطات لمياه الشرب في بغداد و التشخيص الجزيئي لبعض الطحالب الخضر المزرقة المنتجة للسم

مقدم أطروحة جامعية

Abd, Ibrahim Jabir

مشرف أطروحة جامعية

Jawad, Abd al-Latif Muhammad

أعضاء اللجنة

Shathir, Muhammad F.
Abbas, Ithar Kamil
Shawkat, Muayyad Sabri
al-Judi, Nimat Jamil Abd al-Baqi
Alqam, Fuad Munhar

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

The quality of drinking water may be significantly reduced by the presence of cyanobacteria capable of producing toxins.

from September 2011 to August 2012, samples of water were taken monthly from three drinking water treatment stations (Sharek Dijla, Al- Wathba and Al-Rasheed) located on Tigris River in Baghdad area at three sites included river intake, sedimentation tank (phytoplankton) and walls of sedimentation tank (periphyton) .

A total of 268 algal taxa were identified from all collected samples from the studied stations, 161 taxa belonged to Bacillariophyceae (diatoms), 58 taxa to chlorophyceae and 49 taxa to cyanophyceae.

The total count of attached cyanophaceae (periphyton) was the hgihest in Al- Wathba and Al-Rasheed stations compard with Bacilariophyceae, while total count of cyanophyceae in sedimentation tank was the lowest.

Seven species of blue-greens were isolated from the river intake of the studied stations which are: Microcystis aeruginosa, Lyngbya sp., Microcystis flos- aquae, Chroococcus turigidus, Westiellopsis prolifica, Oscillatoria limnetica and Nostoc carneum which belonged to four cyanobacterial orders: Oscillatoriales, Chroococales, Stigonematales and Nostocales.

These genera were dominated in three stations in most of the study period.

Westiellopsis prolifica was identified as new record species in Iraq belonged to the order of Stigonematales.

Two sets of primer were used in molecular analysis for detection of cyanobacteria and microcystin producing isolates.

The first set of primers (PCβF/PCαR) confirmed the presence of cyanobacterial DNA from isolates collected from fresh water of studied stations and amplified a 650 bp gene fragment from the phycocyanin that shared by all cyanobacteria.

The second set of primer (HEPR/HEPF) belonged to myc E gene was successfully amplified and showed 472 bp fragments in gel electrophoresis from all tested hepatotoxic species.

This study was aimed to detect the toxigenic cyanobacteria in three drinking water treatment stations by using traditional and molecular analysis.

التخصصات الرئيسية

الأحياء

الموضوعات

• علم الأحياء الدقيقة
• علم السموم
• المياه
• مياه الشرب
• العراق

عدد الصفحات

151

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-598804