Potential Impacts of uranium pollution and its relation to some immunological parameters in a sample of Iraqi patients with lung cancer

العناوين الأخرى

التأثيرات المحتملة للتلوث باليوررانيوم و علاقته ببعض المؤشرات المناعية في عينة من المرضى العراقيين المصابين بسرطان الرئة

مقدم أطروحة جامعية

al-Rabii, Asil Ismail Ibrahim

مشرف أطروحة جامعية

Salih, Duha Sad
Shanshal, Muthanna Abd al-Jabbar

أعضاء اللجنة

Shafiq, Shafiq S.
Ali, Sawsan S. Muhammad
al-Thuwayni, Aminah Nimah
al-Jilawi, Majid Husayn
al-Azzawi, Muhammad Nafi Ali

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

Human health is affected badly by all types of environmental pollution including : physical, chemical, and biological.

As a result are finding an increase in the incidence of cancer, congenital deformities, and metabolic diseases.

Iraqi environment has been exposed to these pollutants during the last three decades.

The consequences should be investigated, and solutions should be suggested.

Immune system is defending the human body against all types of diseases ; the innate immunity is affected by all pollution factors including uranium pollution.

In order to know whether immune system of Iraqi people is affected or not, the present study was conducted to evaluate some innate immunity parameters namely Natural Killer cells (NK) and Interferon gamma (IFN-γ) beside evaluation of DNA damage.

In this study 110 subjects were included and classified into four groups :  First Group (G1), including 30 subjects of lung cancer patients / Radiation and Nuclear Medicine Hospital / Baghdad.

 Second group (G2), including 29 healthy subjects working in Geological Survey Department / Ministry of Industry, (Positive control).

 Third group (G3), including 20 healthy subjects of Al-Tuwaitha residents, (Positive control).

 Fourth group (G4), including 31 healthy subjects reside in different parts of Bagdad city, (Negative control).

Blood and urine samples were collected from all subjects during the period September 2012 to April 2013.

Each blood sample was divided into two portions: the first used for Natural Killer estimation, the other used for IFN-γ estimation in serum.

Sera were kept at -80C˚ till before using.

Urine samples as well were divided into two portions: the first was used for uranium pollution estimation, the second was used for the estimation of 8- Hydroxydeoxyguanosin (8-OHdG) which reflects DNA damage.

Urine samples were centrifuged by 1000 g/ 15 min, and the supernatants were kept frozen at -80C˚ till using.

Uranium pollution was estimated using Solid State Track Nuclear Detector (SSTND) technique (CR-39 and LR-115).

Natural Killer cells were estimated applying Flow Cytometer technique.

Enzyme Linked Immunosorbent Assay (ELISA) was used for estimation of IFN-γ concentration in serum and 8-OHdG concentration in urine samples.

Uranium concentration was found above the reference value (300 ng / L ; WHO- 2004) in 36.7% of group G1, 13,8% of group G2, 5% of group G3, while it was lower than the reference value in the group G4.

Natural Killer cells percentage was within normal values (5-15%) in 13.3% of the first group G1, 25% of the fourth group G4, while no subject involved in the second and third group had NK estimation within the normal value.

IFN-γ concentration was 0.125 pg/ml for all subjects which represent the lowest measurable value according to the standard of the technique.

Concentration of 8-OHdG compound was statistically highest in the first group (G1) 637.08 ± 48.07 ng/ml, while the second and third group (positive control) appeared statistically highest than the fourth group (negative control) 466.93 ± 53.60 ng/ml , 326.74 ± 90.66 ng/ml, and 283.90 ± 43.14 ng/ml for group G2, group G3, and group G4 respectively.

Low dose chronic uranium pollution seems to be present.14.5% of all subjects have uranium concentration above reference value 300 ng / L according to WHO-2004.

95% of healthy control subjects have innate immune suppression according to results of NK cells percentage.

All subjects have low level of IFN-γ, which may reflect natural immune suppression in all healthy controls too.

DNA damage was high in the Positive control (group G2) as compared with Negative control (group G4).

التخصصات الرئيسية

الطب البشري

الموضوعات

• المرضى
• الرئتان
• السرطان(مرض)
• العراق
• التشخيص

عدد الصفحات

98

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-598963