Cytogenetic and cytotoxic studies on the effect of phytoinvestigated active compounds of Hyoscyamus niger (in vivo and ex vivo)‎

العناوين الأخرى

دراسة وراثية خلوية و سمية حول تأثير المركبات الفعالة لنبات البنج داخل و خارج الجسم الحي

مقدم أطروحة جامعية

Ismail, Asma Ubayd

مشرف أطروحة جامعية

al-Hassani, Hayfa Hadi
al-Samirrai, Khulud Wahib Abbud

أعضاء اللجنة

Ahmad, Salman A.
al-Faysal, Abd al-Husayn Muwayt
Jasim, Hamid Majid
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
Ibrahim, Kazim Muhammad

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم التقانة الإحيائية

دولة الجامعة

العراق

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2011

الملخص الإنجليزي

In this study, different extracts of Hyoscyamus niger were detected using different chemical methods.

Alkaloids were represented the major compound in thus plant, this, it selected for further study.

Genetic effects of alkaloidal extract on mice were determined using several parameters such as mitotic index (MI); micronuclei assay (MN) and chromosomal aberrations (CAs).

The results showed increasing mitotic index and decreasing the spontaneous frequency of chromosomal aberrations and micronuclei.

On the other hand, the free radicals scavenging activity of seven fractions of alkaloidal extract were evaluated by 2, 2-diphenyl-1- picrylhydrazyl (DPPH) assay.

Only one fraction of alkaloidal extract exhibited moderate free radical scavenging activity in comparison with the positive and negative controls.

The cytotoxic activity of different concentrations of alkaloidal extract of H.

niger on cancer cell lines (Hep- 2, AMN-3, RD) estimated at various period of incubation (24, 48, or 72hrs).The effect of this extract was time and concentration-dependant. Remarkably, alkaloidal extract at 1000 μg\ml achieved a significant cytotoxicity on Hep-2 after 72 hrs of incubation period.

Furthermore, viability assay was used to assess the cytotoxic effects of this extract on other types of cell lines (PC-3, HepG2, A549 and WRL68), obtained from Malaysia centre for natural product research and drug discovery(MCNPRD), university of Malaya, It was noticed that low decrease in cell viability of cell lines used above.

In addition, the apoptotic activity of this extract on cancer cells A549 and PC-3 were examined by using multiparameter 3 kits; it was observed a changing in nuclear size, morphology, and cell membrane permeability, mitochondrial membrane potential and releasing of cytochrome C.

التخصصات الرئيسية

الأحياء
النبات

الموضوعات

• علم الوراثة

عدد الصفحات

70

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-600841