Production and purification of alpha hemolysin produced from local isolate of Serratia marcescens
مقدم أطروحة جامعية
مشرف أطروحة جامعية
al-Tai, Maha Fakhri
Hamzah, Subhi Jawad
أعضاء اللجنة
al-Nida, Sad M.
Kazim, Khalid Jabir
الجامعة
جامعة بغداد
الكلية
كلية العلوم
القسم الأكاديمي
قسم التقانة الإحيائية
دولة الجامعة
العراق
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2014
الملخص الإنجليزي
Twenty isolates that have been identified as Serratia marcescens were collected from infected urinary tract infection (UTI).
These isolates were found to produce hemolysin as indicated by clear zone around colonies grown on blood agar plates.
Isolates were screened for their production and the highest producing isolate was selected for further experiments.
Hemolysin was completely precipitated using 50-75% saturation of ammonium sulphate.
Then followed by ion exchange chromatography with DEAE-cellulose, The hemolysin was not detected in the wash step, while in elution step, only one of the two peaks showed hemolytic activity by measuring the absorbance of hemoglobin at 405 nm and as much as 29 μg/ml of hemolysin was quantified.
Finally hemolysin was completely purified to estimate the molecular weight which was shown to be 45 KD by using gel filtration chromatography using sepharose 4B, and hemolysin concentration was reached to 22 μg/ml.
Subsequently, three doses of hemolysin (1, 5 and 10)μg/ml were given orally for seven days to experimental animals (mice) in order to measure the cytogenetic effects of this toxin using three parameters, (mitotic index (MI), micronulcleus (MN) and chromosomal aberrations (CAs) in comparison with phosphate buffer saline (PBS) as a negative control and mitomycin C (MMC) as a positive control.
The results of the effects of the three doses of hemolysin on mice indicated that all doses caused a significant decrease in MI which were shown to be (1.10%, 0.71 % and 0.32%) compared with negative control (1.742%) and positive control (0.742%).
Moreover, a significant increase in the percentage of MN was observed to be (1.92%, 2.88% and 4.04%) in comparison with negative control (0.16%) and positive control (4.132%).
Also a significant increase was seen in the values of CAs which were (0.744, 1.006 and 1.480 %) compared with negative control (0.244% ) and positive control ( 1.498%).
التخصصات الرئيسية
الموضوعات
عدد الصفحات
104
قائمة المحتويات
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussions.
Conclusions and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Ajili, Amir Qani Kazim. (2014). Production and purification of alpha hemolysin produced from local isolate of Serratia marcescens. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601072
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Ajili, Amir Qani Kazim. Production and purification of alpha hemolysin produced from local isolate of Serratia marcescens. (Master's theses Theses and Dissertations Master). University of Baghdad. (2014).
https://search.emarefa.net/detail/BIM-601072
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Ajili, Amir Qani Kazim. (2014). Production and purification of alpha hemolysin produced from local isolate of Serratia marcescens. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601072
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-601072
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر