The effect of colloidal silver concentration and metallic copper against some hospital acquired infections isolates

العناوين الأخرى

تأثير تركيز محلول الفضة الغروي و النحاس المعدني في بعض أنواع البكتريا المعزولة من المستشفيات

مقدم أطروحة جامعية

Hadi, Alya Muhammad

مشرف أطروحة جامعية

Melconian, Alice Krikor

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم التقانة الإحيائية

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

This study is an attempt to prove that metallic copper and colloidal silver solutions have antimicrobial activities against different bacterial species which are considered as a major cause of nosocomial infections .

One hundred and twenty samples were collected from different clinical sources ( urine , blood , pus , sputum , stool and wound swabs ) during the period from October /2012 to December /2012 from different hospitals in Baghdad including AL-Yarmouk teaching hospital and Baghdad teaching hospital .

According to the collected samples , bacterial isolates were distributed as ( 22 ) Escherichia coli isolates ( 18.3%) , ( 12 ) Pseudomonas aeruginosa isolates( 10%) and ( 14 ) Staphylococcus aureus isolates ( 11.6% ).

All these isolates were identified according to morphological and biochemical characteristics by using conventional methods and VITEK2 compact system for confirmation .

The susceptibility test of the bacterial isolates was determined using (17) antimicrobials for E.coli isolates , ( 15 ) antimicrobials for P.aeruginosa isolates and ( 19 ) antimicrobials for S.aureus isolates .

All these antimicrobials used were detected by using VITEK2 compact system .

The results showed variation in resistance to the different antimicrobials tested on bacterial isolates .

From each bacterial species three bacterial isolates with the highest resistance level were selected for this study .

Since the bacterial species E.coli , P.aeruginosa and S.aureus are considered both as a major cause of nosocomial infections and as the most common species persisted in the clinical samples , these therefore were chosen to(1) evaluate the effectiveness of the metallic copper to reduce the viability of the selected bacterial isolates compared with the effect of both aluminum and stainless steel .

(2) to evaluate both the susceptibility of the bacterial isolates to different concentrations of colloidal silver solutions using the agar-well diffusion method and study its effect on the viability reduction of bacterial isolates by using plate-viable count.

An inoculum of ( 107 CFU/ml ) from each of the selected bacterial isolates was inoculated onto coupons ( 1cm x 1 cm ) of copper , aluminum and stainless steel Summary II ________________________________________________________________ and incubated at room temperature for various time periods ranging from 30 to180 minutes.

Viability was determined using the plate -viable count method.

Copper results revealed a complete kill for tested bacterial isolates after 120 minutes of exposure at room temperature indicating a high significant rate of viability reduction(P<0.0001) .

In contrast , viable bacterial colonies were detected on aluminum and stainless steel coupons after 180 minutes of exposure .

The results showed that the tested isolates were sensitive to colloidal silver solutions at both concentrations.

The killing rate studies for various time periods ranging from 30 to 180 minutes at room temperature using plate viable count showed that colloidal silver was highly bactericidal against the tested bacterial isolates at both concentrations .

Complete killing was achieved by 10 ppm concentration after( 180 ) minutes of exposure , which indicates high significant (P<0.0001) in viability reduction .

التخصصات الرئيسية

الأحياء

عدد الصفحات

136

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-601164