Immunochemical and genetic study on Iraqi women with recurrent abortion

العناوين الأخرى

دراسة كيميائية مناعية وراثية للإسقاط المتكرر في العراق

مقدم أطروحة جامعية

Shallal, Sarah Sabri

مشرف أطروحة جامعية

al-Azzawi, Hasan Fayyd Samir

أعضاء اللجنة

al-Saffir, Jinan M. J.
Salih, Shahlaa M.
al-Qazzaz, Abd al-Karim A.

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم التقانة الإحيائية

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

This study was designed to search for some causative and risk factors in 50 aborted women who had history of spontaneous recurrent abortion as case group compared with 30 healthy pregnant women as control group in two hospitals (Al-Elwiya and Al-yarmouk teaching hospitals) in Baghdad city from December 2012 till June 2013.

Four aspects were involved in this study; the first aspect included the measurement of sera antibodies (IgG& IgM) for Toxoplasma gondii cytomegalovirus and rubella infection in aborted and normal pregnant women.

The second aspect determined the levels of sera anticardiolipin and antiphospholipid (IgG&IgM) in the sera of aborted and normal pregnant women.

The third aspect focused on evaluation the levels of the protein C, protein S and activated protein C resistance, in addition to homocysteine levels in the sera of aborted and normal pregnant women, while the fourth aspect studied the association between the polymorphisms of methylenetetrahydrofolate reductase gene in Iraqi women with recurrent abortion compared with normal pregnant women by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).

Results showed the seroprevalence (IgG) toxoplasmosis (latent infection) was 48% in RSA group, while for (IgM) toxoplasmosis (recently acquired infection) was 4% and seroprevalence (IgG) of cytomegalovirus was 14% in RSA group, while the seroprevalence (IgM)) was 4%.Seroprevalence (IgG) of rubella was 12% in RSA group, and 4 % for IgM rubella antibody while in the control group, 6.6% were positive for IgG and IgM antibodies to Rubella.

Results showed increased in positive rates of total شnticardiolipin antibody (ACLA) (48%) in abortion group which were very significantly higher compared with that in the control group (P<0.01).

Thirty percent with recurrent pregnancy loss cases showed high levels of homocysteine levels in the studied populations.

A significant decrease in Protein C levels (33.3%) of recurrent abortion group as compared with (3.3%) in control group.

Activated protein C resistance incidence was higher in abortion women group compared with the control group.

Antiphospholipid antibodies were positive in 20% of abortion cases and none in controls.

To determine the association of (C677T and A1298C) of the Methyltetrahydrofolate reductase (MTHFR) gene mutations with RSA, total genomic DNA was isolated from blood leukocytes.

The frequency of the two common C677T and A1298C MTHFR gene mutations in the patients and controls was determined using PCR-restriction fragment length polymorphism (PCR-RFLEP).

Results showed no significant difference in the prevalence of 677C/T genotype among women with RSA and healthy controls (P = 0.285).

Also no statistically significant difference in the frequency of A1298C MTHFR gene mutation was detected between the two groups (P=0.175).

In conclusion, the results also showed no significant difference in MTHFR C677T/A1298C genotype in Iraqi women with recurrent spontaneous abortion.

التخصصات الرئيسية

الأحياء

الموضوعات

• الإجهاض
• الحمل
• العراق

عدد الصفحات

117

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-601214