Construction of nanobiosensors for detection of biomarkers

العناوين الأخرى

بناء متحسسات حيوية نانوية للكشف عن المؤشرات الحيوية

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم التقانة الإحيائية

دولة الجامعة

العراق

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

The present study was designed to detect both CA125 and glucose biomarkers by using nanoparticles based sensor.

An enzyme-linked immunoassay based on dual signal detection has been developed for the detection of ovarian cancer biomarker CA125.

The sandwich-type immunoassays use nanoelectrode array (NEA) chip and absorbance methods for the dual detection.

The NEA is used to confirm the optical detection of CA125 that was carried out in a high-binding 96-well plate.

An alkaline phosphatase (AP) enzyme was used to label the detection antibody to allow both the optical and electrochemical identification.

Two kinds of substrates were catalyzed by the AP enzyme.

Para-nitrophenylphosphate (PNPP) produces chromogenic para-nitrophenol (PNP), which can be optically detected at 405 nm.

Para-aminophenylphosphate (PAPP) produces electroactive para-aminophenol (PAP), which can be detected amperometrically between -0.1 to 0.3 V.

The linear range has been determined to be 5-1000 U/ml for both the optical and electrochemical immunoassays.

The limit of detection (LOD) of the optical immunoassay was determined to be 1.3 U/ml and for the NEA amperometry was 40 U/ml.

The low LOD for both transduction methods are promising for early stage detection of cancer through CA125 which has a physiological level at 35 U/ml.

For analytical glucose determination a surface enhanced Raman spectroscopy (SERS) coupling with GOx-catalyzed oxidation of glucose were applied to evaluate glucose concentration by measuring Raman intensity for hydrogen peroxide (H2O2) that produced from glucose oxidation by GOx enzyme.

Under optimized conditions the calibration curve was established.

The linear regression curve was plotted using y = 1575x + 1109 with very good correlation (R2 = 0.97 ) , recorded after 10 min .Besides, the limit of detection (LOD) was 0.278 mM.

The reliability of the sensor was confirmed with the most important interferences in blood ascorbic acid (AA) and uric acid (UA), the assay did not show any interference with the desired signal.

Ultimately, saliva was used as analytical fluid to sense concentration of glucose instead of using finger prick.

Application of saliva for detecting glucose revealed promising results with more comfortable way for patients.

التخصصات الرئيسية

الأحياء

عدد الصفحات

106

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Introduction.

Chapter One : Literature review.

Chapter Two : Materials and methods.

Chapter Three : Results and discussions.

Conclusions and recommendations.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Aqaidi, Isra Ali Zaydan. (2013). Construction of nanobiosensors for detection of biomarkers. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-601381

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Aqaidi, Isra Ali Zaydan. Construction of nanobiosensors for detection of biomarkers. (Doctoral dissertations Theses and Dissertations Master). University of Baghdad. (2013).
https://search.emarefa.net/detail/BIM-601381

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-601381

حفظتم الحفظ طباعة

قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"

أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي

مرصد "معرفة"
لقياس الإنتاج العلمي العربي

تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر