In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds
العناوين الأخرى
الإكثار الدقيق مخبريا لنبات التفاح Malus × domestica من الصنف Anna بزراعة البراعم القيمة و الإبطية
مقدم أطروحة جامعية
مشرف أطروحة جامعية
al-Kishawi, Abbud Yasir
al-Bashiti, Tariq Abd al-Qadir
أعضاء اللجنة
al-Kahlut, Kamal al-Abd Muhammad
Abu Awdah, Muhammad Mahmud
الجامعة
الجامعة الإسلامية
الكلية
كلية العلوم
القسم الأكاديمي
قسم العلوم الحياتية
دولة الجامعة
فلسطين (قطاع غزة)
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2015
الملخص الإنجليزي
Apple plant (Malus x domestica) is perennial fruit tree belonging to the family Rosaceae.
Apple species have wide geographical distribution.
Apple fruit has been considered to have high health benefits.
Most of the species intercross.
Since selfincompatibility is common, apples produced from seed are mostly interspecific hybrids, therefore it is difficult to produce true-to-type cultivars from seeds.
The objective of our study, therefore, was to develop micropropagation protocol for Anna apple (Malus domestica Borkh), which is highly adapted for environmental conditions of Gaza Strip, using buds explants on MS medium.
Buds were collected during the summer season produced higher percentage of buds establishment (75%) as compared to buds collected during other seasons.
The best sterilization protocol was when we used 5% NaOCl, Tween 20, 70% Alcohol and 0.5% HgCl2.
Apple buds (3.0 mm in length) were established by using AC, AA and CA as antioxidants.
In culture establishment we used full strength MS media with several combinations of hormones BAP, GA3 and IBA.
We obtained the best results in group B2 (1mg/l BAP, 0.5mg/l GA3 and 0.1mg/l IBA).
The highest establishment rate was obtained for explant that produced 6.40 ± 1.14 shoots (1.76 ± 0.34 cm in length).
In multiplication stage, we obtained the best results in group H1 (1mg/l BAP, 0.5mg/l GA3 and 0.1mg/l IBA).
The highest multiplication rate was obtained for explant that produced 6.80 ± 3.03 shoots (1.88 ± 0.62 cm in length).
In rooting stage we used two auxins IBA or IAA, the roots were formed only in two samples when we used 1 mg/l IBA and 0.1 mg/l IAA in half strength MS solid media.
This research is considered the first in Gaza Strip for in vitro micropropagation of woody plant by using apple shoot buds.
التخصصات الرئيسية
الموضوعات
عدد الصفحات
98
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusion and recommendation.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abu Saqr, Halah Mahmud Ahmad. (2015). In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-611840
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abu Saqr, Halah Mahmud Ahmad. In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-611840
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abu Saqr, Halah Mahmud Ahmad. (2015). In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-611840
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-611840
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر