In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds
Other Title(s)
الإكثار الدقيق مخبريا لنبات التفاح Malus × domestica من الصنف Anna بزراعة البراعم القيمة و الإبطية
Dissertant
Thesis advisor
al-Kishawi, Abbud Yasir
al-Bashiti, Tariq Abd al-Qadir
Comitee Members
al-Kahlut, Kamal al-Abd Muhammad
Abu Awdah, Muhammad Mahmud
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2015
English Abstract
Apple plant (Malus x domestica) is perennial fruit tree belonging to the family Rosaceae.
Apple species have wide geographical distribution.
Apple fruit has been considered to have high health benefits.
Most of the species intercross.
Since selfincompatibility is common, apples produced from seed are mostly interspecific hybrids, therefore it is difficult to produce true-to-type cultivars from seeds.
The objective of our study, therefore, was to develop micropropagation protocol for Anna apple (Malus domestica Borkh), which is highly adapted for environmental conditions of Gaza Strip, using buds explants on MS medium.
Buds were collected during the summer season produced higher percentage of buds establishment (75%) as compared to buds collected during other seasons.
The best sterilization protocol was when we used 5% NaOCl, Tween 20, 70% Alcohol and 0.5% HgCl2.
Apple buds (3.0 mm in length) were established by using AC, AA and CA as antioxidants.
In culture establishment we used full strength MS media with several combinations of hormones BAP, GA3 and IBA.
We obtained the best results in group B2 (1mg/l BAP, 0.5mg/l GA3 and 0.1mg/l IBA).
The highest establishment rate was obtained for explant that produced 6.40 ± 1.14 shoots (1.76 ± 0.34 cm in length).
In multiplication stage, we obtained the best results in group H1 (1mg/l BAP, 0.5mg/l GA3 and 0.1mg/l IBA).
The highest multiplication rate was obtained for explant that produced 6.80 ± 3.03 shoots (1.88 ± 0.62 cm in length).
In rooting stage we used two auxins IBA or IAA, the roots were formed only in two samples when we used 1 mg/l IBA and 0.1 mg/l IAA in half strength MS solid media.
This research is considered the first in Gaza Strip for in vitro micropropagation of woody plant by using apple shoot buds.
Main Subjects
Topics
No. of Pages
98
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusion and recommendation.
References.
American Psychological Association (APA)
Abu Saqr, Halah Mahmud Ahmad. (2015). In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-611840
Modern Language Association (MLA)
Abu Saqr, Halah Mahmud Ahmad. In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-611840
American Medical Association (AMA)
Abu Saqr, Halah Mahmud Ahmad. (2015). In vitro, micro propagation of an apple Malus × domestica borkh. (Cultivar Anna) by cloning of apical and axillary shoot buds. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-611840
Language
English
Data Type
Arab Theses
Record ID
BIM-611840
