Effect of folic acid, N-acetyl cysteine and insulin and their combinations on uterine histology alloxan induced diabetic pregnant rats and their fetuses

الملخص EN

This study has been carried out to assess the effect of folic acid, N- acetyl Cysteine and insulin on some histological parameters in the uterus of alloxan induced diabetic rats and their fetuses.

In this study, 192 healthy pregnant rat, whose weight was about 200-350grams were used .The animals were housed under standard laboratory condition (12 h light; 12 h dark at 20-24 C).

The animals were given standard rat pellets and tap water ad libtium.

Normal female rats mated with normal males, then the first day of gestation were detected through vaginal plug.

Diabetes mellitus were induced by a single subcutaneous injection of 100 mg/kg of alloxan.

After four days, rats which showed blood glucose of more than 200 mg/dl were considered as alloxan induced diabetic pregnant, and they were distributed to three periods (1-7, 8-14 and 15-22 days) of gestation.

Diabetic pregnant rats at each period were divided into eight subgroups groups.

First subgroup supplemented with standard diet which represent the negative control, while the rest subgroups were treated with 100mg/kg alloxan.

In addition to alloxan, the third subgroup were treated with 250mg/kg folate and 4 I.U .insulin, the subgroup four was daily injected with 7.5mg/kg folate and 4 I.U.

insulin, the subgroup five was supplemented with 250mg/kg folate, subgroup six was daily injected with 4 I.U.

insulin, subgroup seven was supplemented with 0.1 %N-acetyl cysteine and 4 I.U .insulin and the rats of the last group were supplemented with 0.1 %N-acetyl cysteine.

Histological examination of uteri sections revealed thickness and of uterine wall hyperplasia and stenation of the uterine mucosa in all treated groups during the first week of gestation .Infiltration of the uterine wall with eosinophilic cells were seen in folic acid (250 mg/hg) treated subgroup .Growing embryos were seen during the second week of gestation and full grown embryos were seen during the third week of gestation in all treated groups.

Abnormal development brain seen in fetuses of diabetic rats.

Also abnormal and restricted of peripheral area around the brain were seen in insulin treated rats.