Biochemical and physiological effects of melatonin, vitamin e + selenium on heart, lung and kidney antioxidant enzymes of male aging rats

الملخص EN

Free radicals are one of the potential major causes of age related destruction.

They are defined as any atom or molecule that possesse one or more unpaired electron.

Antioxidants such as vitamin E+ Selenium and melatonin are substances that prevent deterioration damage caused by free radicals.

They have been used in prophylaxis and treatment of various diseases affect elderly persons include cancer, cardiac diseases multiple sclerosis and arthritis.

In this study 48 male albino rats were kept at constant environmental and nutritional conditions, then classified into 4 equal groups.

Group I used as control young (2-4 months old) not supplied by any additives, group II control aging (12-27 months old) not supplied by any additives, group III aged male rats administered by melatonin at a dose 0.27 mg/kg body weight daily for three months orally, and group IV aged rats injected quickly by I.M route with vitamin E+ Selenium 20 mg/kg body weight and 0.15 mg/k.

gm.

b.

w, respectively for three months.

After 12 weeks from the onset of administration the rats were killed by decapitation.The heart, lung and kidney were rapidly removed, washed with saline and processed directly for determination of superoxide dismutase activity (SOD), Glutathione peroxidase (GSHPX), catalase activity and Reduced glutathione (GSH).

The obtained data revealed significant decrease in heart, lung and kidney (SOD) and (GSHPX) activities of aged male rates.

A high significant increase in heart and lung (SOD), kidney (GSHPX) activities as well as significant increase in (GSHPX) of heart and lung in melatonin administered group.

Moreover significant increase in heart and lung (SOD) and heart, kidney (GSHPX) of aged male rats treated with vitamin E+ selenium.

After administration of melatonin, heart and lung catalase activity increased significantly while (GSH) concentration showed significant decrease in kidney of aged rates, significant increase in heart and kidney of melatoin administrated group, significant increase in heart of vit.

E+ selenium injected group.