Compartive study between the effects of human cd34 and rat bone marrow mesenchymal stem cells on amelioration of ccl4 induced liver fibrosis

الملخص EN

Human umbilical cord blood (UCB) cells and rat bone marrow mesenchymal stem cells (BM-MSCs) have many advantages as grafts for cell transplantation.

The aim of this study was to investigate the impact of UCB cells and BM-MSCs on reversal of hepatic injury and revival of hepatic function in a rat model of carbon tetrachloride (CCl4)-induced liver fibrosis.

Keywords: CCl4 liver fibrosis, Umbilical cord blood CD34＋, Bone marrow stem cell, Stem cell therapy.

Stem cells administration recovered liver function that confirmed by histopathological examination of liver tissue.

GFP labeled cells were detected in groups injected with stem cells.

Concerning gene expression, stem cells administration reduced gene expressions of collagen 1, TGF-1, and TNF- with lower expression in CCl4/CD34＋group.

Albumin, MMP-2 and MMP-9 genes were highly expressed in liver tissues in the groups that received stem cells with higher expression in the group that received undifferentiated CD34+.

Human UCB CD34＋ stem cells were more effective in alleviation of CCl4-induced liver injury in rat than BM-MSCs.

This study highlights an important role of human UCB CD34＋ stem cells in liver fibrosis therapy.