التوصيف الجزيئي و المظهري لبعض أنواع القرنفل

الملخص EN

The study included two phases, the first one was carried out at one of Baghdad nurseries on 15/9/2013 sowing the seeds of 12 species of Dianthus spp.( D.

caryophyllus, D.

chinensis, D.

gratianopolitanus, D.

barbatus, D.

balbisii, D.

repens, D.

alpinus, D.

monspessulanus, D.

pavonius, D.

deltoids, D.

japonicas and D.

seguieri) to study some of the qualities phenotypic, and the second phase was carried out at biotechnology and tissue culture laboratories /College of Agriculture / University of Razi / Kermanshah / Iran .

The Amplified Fragment Length Polymorphism (AFLP) based on Polymerase Chain Reaction (PCR) with seven primers was implemented.

The results of the phenotypic characteristics of the species studied, and there are considerable differences between them where the variation coefficient (C.V.)was very high in all the traits and reached the top rated 85.09 for a number of branches /plant , 57.66 for the number of flowers / plant, 45.08 flower diameter, 32.51 for plant height and 28.66 dry weight of vegetative part .

DNA analysis showed that the primers gave a 397 bands including 324 polymorphic bands and 81.59% polymorphic bands , this confirms the existence of Genetic Distance between the species of Dianthus studied .

High Genetic Distance 0.28% D.

barbatu and D.

caryophyllus and lowest Genetic Distance0.08% between D.

chinensis and D.

japonicas.