Vitrification and droplet-vitrification cryopreservation for wild artemisia (artemisia herba-alba Asso.)‎ shoot-tips

الملخص EN

This study shows the effect of conserving shoot-tips of Artemisia herba-alba via two vitrification based cryopreservation techniques (Vitrification and droplet-vitrification).

In vitrification three separate experiments were conducted to study the effect of loading and cryoprotectant solutions and their concentrations on the survival and regrowth of Artemisia shoot-tips, The simplest efficient cryopreservation vitrification dependent technique of Artemisia herba-alba shoot tips was by using two-step increased concentration of PVS2 as it gave high survival (89%) and regrowth (81%) percentages after freezing.

In droplet vitrification the shoot-tips were vitrified with chilled plant vitrification solution and placed on aluminum foil strips prior to plunge in liquid nitrogen.

Dropletvitrification technique was suited method for conserving Artemisia herba-alba shoot tips giving high survival (93.3%) and regrowth (73.3%) after 35 min of exposure to PSV2 and after plunge in liquid nitrogen.