Molecular characterization of a duck virus hepatitis isolate isolated from Sharkia Governorate
العناوين الأخرى
الوصف الجزيئي لمعزولة التهاب كبدي فيروسي من البط بمحافظة الشرقية
المؤلف
Muhammad, Hisham Ahmad Abd al-Badi
المصدر
Assiut Veterinary Medical Journal
العدد
المجلد 61، العدد 147 (31 أكتوبر/تشرين الأول 2015)، ص ص. 56-65، 10ص.
الناشر
تاريخ النشر
2015-10-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In the present study a Blencher duckling flock with 70% recurrent mortality on each reared batch was examined for the cause of mortality.
live duckling were showing nervous signs and opisthotonos, sacrificed samples revealed depressed areas on the liver marking the large blood vessels while Hemorrhagic lesion suggestive for DVH were only noticed on liver of dead duckling carcasses.
Liver samples were collected for virus isolation trials and for immunofluorescence.
Initial virus isolation in embryonated chicken eggs ECE after ultra-filtration through 400 nm membrane filter revealed small size hemorrhagic edematous embryos this lesion was consistent upon a series of ultra-membrane filtration through 200 and 100 nm membrane filters which indicate a Picorna virus.
Immunofluorescence examination revealed positive results for duck virus hepatitis (DVH).
Clinical samples examined by generic RT-PCR assays followed by partial sequence analysis of the 3D gene revealed that the isolate was characterized as Duck hepatitis A virus resembling the strain (DHV/Duck / Egypt / Al-Gharbia /2014) in the 3D protein gene whom its gene bank accession number was (KP202874).
The current study stresses on the value of ultra-membrane filtration through a 100 nm membrane filters as a rapid diagnostic tool For DVH without the need for extra laboratory diagnostic work.
Interpretation of sequence results revealed that.
The isolated (DVH/EG.
Bayoumieh-Sharkia-2015) is a Duck hepatitis type A virus.
With 100% resemblance to the strain (DHV/Duck/ Al-Gharbia /2014) Egypt/ in 3D protein gene with gene bank accession number (KP202874) unpublished data.
Sequence and phylogenetic analysis indicated that the (DVH/EG.
Bayoumieh-Sharkia-2015) isolate is clustered in the DHAV serotype 1 but was distinguishable from the other isolated Egyptian strains with resemblance ranging between 63.1 - 63.8%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Muhammad, Hisham Ahmad Abd al-Badi. 2015. Molecular characterization of a duck virus hepatitis isolate isolated from Sharkia Governorate. Assiut Veterinary Medical Journal،Vol. 61, no. 147, pp.56-65.
https://search.emarefa.net/detail/BIM-648731
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Muhammad, Hisham Ahmad Abd al-Badi. Molecular characterization of a duck virus hepatitis isolate isolated from Sharkia Governorate. Assiut Veterinary Medical Journal Vol. 61, no. 147 (Oct. 2015), pp.56-65.
https://search.emarefa.net/detail/BIM-648731
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Muhammad, Hisham Ahmad Abd al-Badi. Molecular characterization of a duck virus hepatitis isolate isolated from Sharkia Governorate. Assiut Veterinary Medical Journal. 2015. Vol. 61, no. 147, pp.56-65.
https://search.emarefa.net/detail/BIM-648731
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 64-65
رقم السجل
BIM-648731
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر