Effect of zinc and EDTA on lead and cadmium residues in buffalo calves

الملخص EN

This study was conducted to investigate the ameliorative effect of dietary supplementation of either Zn or EDTA or zinc+EDTA on reducing plasma and tissue residues using 20 buffalo calves' naturally exposed to environmental lead (Pb) and cadmium (Cd) reared in Mahlet-Mosa Farm in Kafr El–Shiekh governorate.

Lead and cadmium levels were quantified fortnightly in blood samples throughout the fattening period (6 month) and the residues in liver, kidney and eye muscles after slaughtering were measured.

It was found that the additions of Zn, EDTA and zinc+EDTA reduced the level of Pb in blood by 37, 42 and 20%, respectively; the corresponding residues of cadmium in plasma were 37.5, 50 and 25%, respectively compared to the control group.

Also, the treatments effectively reduced (P<0.05) the lead and cadmium residues in soft tissues (liver, kidney and eye muscles).

The levels of lead in liver were lower (P<0.05) in EDTA, zinc and zinc+EDTA treated groups (0.27, 0.20 and 0.31ppm), than the control group 0.55ppm.

Meanwhile, the residues of lead in kidney were varied (P<0.05) among the different treatments being 0.17, 0.21 and 0.45ppm in EDTA, zinc and EDTA+zinc treated groups, receptively, compared to 0.75ppm in the control group.

The residues of lead in the eye muscle were 0.08, 0.06 and 0.11ppm for zinc, EDTA and zinc+EDTA groups, respectively, compared to control group (0.15ppm).

The cadmium residues as ppm in liver were less in EDTA (0.18), zinc (023), zinc+EDTA (0.24) treated groups compared to the control group (0.41).

In kidney cadmium residues were ranged between 0.27 to 0.36 ppm in all treated groups.

The levels of cadmium in the eye muscles were lower (P<0.05) in zinc, EDTA and zinc+EDTA (0.02, 0.03 and 0.03ppm, respectively) than the control group (0.05ppm) These results showed that dietary addition of zinc, EDTA and zinc+EDTA has the ability to reduce Pb and Cd residues in blood and soft tissue of buffalo calves without any negative effects.

Moreover, EDTA addtions had more ameliorative Pb and Cd residues the effects of dietary supplementation of either zinc or zinc+EDTA as insured by blood parameters (Hb, PCV, creatinine, urea, AST and ALT) and soft tissues (liver, kidney and eye muscles Longissimus Lumbarium).