Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey

العناوين الأخرى

استخدام أنزيم البيتا غالاكتوزيداز المستخلص من بعض النباتات في تحضير حليب و مصل حليب خالي من اللاكتوز

مقدم أطروحة جامعية

al-Nuaymat, Ali Mudhi

مشرف أطروحة جامعية

Atruz, Umar Muhammad Falih

أعضاء اللجنة

al-Rawashidah, Ibrahim Muhammad
al-Quran, Salih Ahmad
al-Tarawinah, Khalid Ahmad

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2015

الملخص الإنجليزي

Crude β-galactosidase extracts of Zingiber officinale and Alliumn sativum were investigated for the activity β-galactosidase.

The enzyme in the extract was characterized in term of pH, temperature and enzyme kinetic.

The crude β-galactosidase extract of Zingiber officinale and Alliumn sativum used also in hydrolysis of lactose in milk and whey.

The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β -D-galactopyranoside (ONPG).

The crude enzyme extract of Zingiber officinale was found to has maximum activity at pH =5.0 and 60°C.

While extract of Alliumn sativum was found to has maximum activity at pH=4.0 and 500C.

Enzyme kinetics in both extracts were indicated to have a potential activities for example, the km and Vmax values of enzyme extract of Zingiber officinale were 2.08 mM and 0.55µmol/min, respectively while 3.2mM and 1.1 µmol/min, respectively in Alliumn sativum.

The application of crude enzyme extract of both plants for lactose hydrolysis in milk showed a significant decreased in the lactose content 30-45% in raw milk, while 50-60% of lactose hydrolyzed in skimmed milk.

The findings indicate the potential use crude enzyme extract of Zingiber officinale and Alliumn sativum for the production of low/delactosed milk for lactose-intolerant population.

However, 55-60% of lactose hydrolyzed in whey.

These results encourage to use their crude enzyme extract in food industry and in manufacturing syrup sweeteners.

التخصصات الرئيسية

الأحياء
النبات

الموضوعات

عدد الصفحات

44

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results and discussion.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Nuaymat, Ali Mudhi. (2015). Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656343

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Nuaymat, Ali Mudhi. Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656343

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Nuaymat, Ali Mudhi. (2015). Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656343

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-656343