Biodegradation of some 2,4-dinitrotoluene (2,4-DNT)‎ by some bacteria isolated from Maan quarries, Southern Jordan

العناوين الأخرى

التحطم الحيوي لبعض تراكيز 2,4 ثنائي نيترو تولوين بواسطة بكتيريا معزولة من محاجر معان، جنوبي الأردن

مقدم أطروحة جامعية

al-Habahibah, Khalid Atif Muhammad

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad

أعضاء اللجنة

Abu Darwish, Muhammad Sanad
al-Shurafa, Khalid Yusuf Ahmad
al-Shakanbah, Jumah Muti

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2015

الملخص الإنجليزي

This laboratory study were performed to identify and characterize 2,4- DNT degrading bacterial strains isolated from historical explosive contamination soil in Maan quarries, southern Jordan.

The isolation and the identification by using morphological and biochemical characteristics obtain seven bacterial strains, all are monitored in BSM medium for ability to degrade 2,4-DNT as a sole carbon, nitrogen sources by using optical density (OD) for growth, 2,4-DNT disappearances and dropping of pH as indicator for nitrite releases in different concentrations, the results suggested that one strain of total seven bacterial have a high indicator for ability to degrade 2,4-DNT in 110μM concentration , this bacterial strain identification was Pseudomonas oryzihabitans.

Results from the biodegradation experiments by Pseudomonas oryzihabitans indicate that the biodegradation rates of 2,4-DNT with this strain were highly dependent on environmental conditions such as pH and temperature, with optimal conditions obtained at 37 °C and pH ≈7.5.

Supplemental nitrogen and carbon sources in BSM medium are also tested, and the results showed that the maximum degradation rate obtained when BSM medium contain 0.2% (w/v) yeast extract or 0.2% (w/v) glucose respectively, all carbon and nitrogen sources are used except L-proline increase the degradation percentage after ~40 hours of incubation.

However, the BSM with 0.2% glucose or 0.2% succinate as carbon sources have been the highest initial degradation rate respectively, and also the BSM with 0.2% L-serine or 0.2% casein as nitrogen source have been the highest initial degradation rate respectively.

التخصصات الرئيسية

الأحياء

الموضوعات

• علم البيئة
• الأردن
• معان
• البكتيريا

عدد الصفحات

79

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-656416