High concentration of paclitaxel induces partial inhibition and cell death in the human breast cancer cell line mcf-7in-vitro
المؤلفون المشاركون
Salim, Tariq A.
Ismail, Hisham A.
al-Chennawi, Farha A.
al-Rifai, Muhammad
المصدر
Journal of the Egyptian National Cancer Institute
العدد
المجلد 14، العدد 3 (30 سبتمبر/أيلول 2002)، ص ص. 223-228، 6ص.
الناشر
جامعة القاهرة المعهد القومي للأورام
تاريخ النشر
2002-09-30
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
Purpose : cancer remains one of the most common causes of death and thus one of the goals of cancer research has been and continues to be, the discovery of natural and synthetic products for cancer prevention and for treatment.
For time immemorial, plants played a dominant role in the treatment of human ailment.
Therefore, in the present study we examined the in vitro effect of paclitaxel on the growth rate and morphology of the human mammary tumor cell line (MCF-7).
Material and Methods : MCF-7 cells were counted and cultured under aseptic conditions.
Paclitaxel was added to cultured cells at different concentrations, 0.5, 2, 5, 7 and 10 µg / ml.
Both treated and untreated cells were harvested and tested for viability.
Apoptosis was detected by using DAPI fluorescent dye and DNA fragmentation assay.
Results : our results showed that paclitaxel at a concentration of 5µg/ml inhibited in vitro tumor growth significantly within a period of 48 hours Microscopic examination of the cell morphology as well as nuclear staining revealed that the paclitaxel treated-cells suffered dramatically from membrane swelling, disruption and nuclear fragmentation.
Conclusion : Necrosis and apoptosis seem to be the underlying mechanisms by which paclitaxel affect human mammary tumor cells in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Rifai, Muhammad& Salim, Tariq A.& Ismail, Hisham A.& al-Chennawi, Farha A.. 2002. High concentration of paclitaxel induces partial inhibition and cell death in the human breast cancer cell line mcf-7in-vitro. Journal of the Egyptian National Cancer Institute،Vol. 14, no. 3, pp.223-228.
https://search.emarefa.net/detail/BIM-67485
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Salim, Tariq A.…[et al.]. High concentration of paclitaxel induces partial inhibition and cell death in the human breast cancer cell line mcf-7in-vitro. Journal of the Egyptian National Cancer Institute Vol. 14, no. 3 (Sep. 2002), pp.223-228.
https://search.emarefa.net/detail/BIM-67485
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Rifai, Muhammad& Salim, Tariq A.& Ismail, Hisham A.& al-Chennawi, Farha A.. High concentration of paclitaxel induces partial inhibition and cell death in the human breast cancer cell line mcf-7in-vitro. Journal of the Egyptian National Cancer Institute. 2002. Vol. 14, no. 3, pp.223-228.
https://search.emarefa.net/detail/BIM-67485
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
includes bibliographical references : pp. 227-228
رقم السجل
BIM-67485
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر