The siRNA-mediated down-regulation of vascular endothelial growth factor receptor1
المؤلفون المشاركون
Sani, Muslim Jafari
Yazdi, Fuad
Karimi, Masumah Masumi
Ali Zadah, Javad
Rahmati, Majid
Mahmud Abadi, Ali Zarei
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 18، العدد 4 (30 إبريل/نيسان 2016)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2016-04-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
الملخص EN
Background: Angiogenesis is an important biological process involved in the proliferation of endothelial cells, tumor growth and metastasis.
Vascular endothelial growth factor (VEGF) is considered as a prominent regulator of angiogenesis which exerts the aforementioned effect(s) through its respective receptors (VEGFR1 and VEGFR2).
VEGF receptors are targeted as a therapeutic candidate for cancer growth inhibition.
RNAi as a new and promising strategy has provided a useful means to specifically suppress gene expression in cancer cells.
Objectives: The current study aimed to down-regulate expression of the VEGFR1 using siRNA.
Materials and Methods: This experimental study designed specific siRNAs against VEGFR1.
Total RNA was extracted from human umbilical vain endothelial cell (HUVEC) and subsequently cDNA was synthetized.
PCR was performed using specific primers to amplify the target gene.
After double digestion and purification, the gene was cloned into pEFGP-N1 expression vector.
Then, AGS cells were transfected with recombinant pEGFP-N1 using lipofectamin.
The gene expression and down-regulation were evaluated by fluorescence scanning, reverse transcription PCR (RT-PCR) and Western blot techniques.
Results: Fluorescent scanning, RT-PCR (27.68%) and western blot analysis (31.06%) showed that the expression of VEGFR1 was suppressed effectively.
Conclusions: The results of the current study showed that specifically designed siRNA can be considered as an appropriate strategy to suppress gene expression and might be a promising tool to prevent angiogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sani, Muslim Jafari& Yazdi, Fuad& Karimi, Masumah Masumi& Ali Zadah, Javad& Rahmati, Majid& Mahmud Abadi, Ali Zarei. 2016. The siRNA-mediated down-regulation of vascular endothelial growth factor receptor1. Iranian Red Crescent Medical Journal،Vol. 18, no. 4, pp.1-7.
https://search.emarefa.net/detail/BIM-679528
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sani, Muslim Jafari…[et al.]. The siRNA-mediated down-regulation of vascular endothelial growth factor receptor1. Iranian Red Crescent Medical Journal Vol. 18, no. 4 (Apr. 2016), pp.1-7.
https://search.emarefa.net/detail/BIM-679528
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sani, Muslim Jafari& Yazdi, Fuad& Karimi, Masumah Masumi& Ali Zadah, Javad& Rahmati, Majid& Mahmud Abadi, Ali Zarei. The siRNA-mediated down-regulation of vascular endothelial growth factor receptor1. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 4, pp.1-7.
https://search.emarefa.net/detail/BIM-679528
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-679528
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر