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Serum sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of patients with membranous nephropathy and focal and segmental glomerulosclerosis
المؤلفون المشاركون
Pant, Pragya
Singh, Santosh K.
Singh, Vijay P.
Doley, Prodip Kumar
Sivasankar, M.
Usha, N. I.
Singh, R. G.
المصدر
Saudi Journal of Kidney Diseases and Transplantation
العدد
المجلد 27، العدد 3 (30 يونيو/حزيران 2016)، ص ص. 539-545، 7ص.
الناشر
تاريخ النشر
2016-06-30
دولة النشر
السعودية
عدد الصفحات
7
التخصصات الرئيسية
الموضوعات
- المرضى
- علم الأحياء الجزيئي
- الكلى
- الأمراض
- التحليل الكهربائي
- المتلازمة الكلوية
- التهاب كبيبات الكلى
- التشخيص
- بولي أكريلاميد
- كبريتات لوريل الصوديوم
الملخص EN
Diagnosis of membranous nephropathy (MN) and focal and segmental glomerulosclerosis (FSGS) needs a renal biopsy, which is an invasive procedure with potentially serious complications.
Proteomics may be applied for the development of a biomarker for these diseases which will obviate the need of biopsy.
Serum sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis gives an idea of the various proteins with different molecular weights (MWs) in a given sample.
This study was conducted to analyze proteins with different MWs in patients with MN and FSGS and to compare the two groups with regard to their protein profile.
This was a comparative, experimental study performed from June 2013 to July 2014 in the Department of Nephrology, Sir Sunderlal Hospital, Banaras Hindu University, Varanasi.
Twenty-three histologically diagnosed cases of primary MN and 25 cases of FSGS were included in the study.
Patients were categorized as having mild, moderate, and severe proteinuria with 24 h urinary protein levels of <4, 4– 8 and ≥8 g/24 h, respectively.
SDS-PAGE analysis was performed by the method of Laemmli and revealed a significantly higher number of patients with FSGS (80%) having a protein corresponding to 29 kDa MW, than those with MN (39.1%) (P = 0.004).
Protein of 5 kDa MW was present in a significantly higher number of patients with moderate (80%) and severe (100%) proteinuria than those with mild proteinuria (25%) (P <0.001).
Thus, protein of MW 29 kDa may be a marker for FSGS and needs further characterization.
Similarly, 5 kDa protein, present in patients with moderate and severe proteinuria, might be either contributing to or be a marker of severe illness.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pant, Pragya& Singh, R. G.& Usha, N. I.& Singh, Santosh K.& Singh, Vijay P.& Doley, Prodip Kumar…[et al.]. 2016. Serum sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of patients with membranous nephropathy and focal and segmental glomerulosclerosis. Saudi Journal of Kidney Diseases and Transplantation،Vol. 27, no. 3, pp.539-545.
https://search.emarefa.net/detail/BIM-683634
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pant, Pragya…[et al.]. Serum sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of patients with membranous nephropathy and focal and segmental glomerulosclerosis. Saudi Journal of Kidney Diseases and Transplantation Vol. 27, no. 3 (May. / Jun. 2016), pp.539-545.
https://search.emarefa.net/detail/BIM-683634
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pant, Pragya& Singh, R. G.& Usha, N. I.& Singh, Santosh K.& Singh, Vijay P.& Doley, Prodip Kumar…[et al.]. Serum sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of patients with membranous nephropathy and focal and segmental glomerulosclerosis. Saudi Journal of Kidney Diseases and Transplantation. 2016. Vol. 27, no. 3, pp.539-545.
https://search.emarefa.net/detail/BIM-683634
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 544-545
رقم السجل
BIM-683634
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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