The effect of diode laser 635nm on mitochondrial membrane potential and apoptosis induction of CHO47 cells line

الملخص EN

Several studies suggest that low power laser irradiation is capable of affecting cellular processes under different conditions.In this study, the effect of low-power laser irradiation on CHO47 cells was investigated.The cells were irradiated with a CW, 635 nm Diode laser at an energy density ranging from 2.94 to 41.16 J / cm2 (power output, 100mW).

The cells were grouped into: group1 control (not irradiated); group 2 exposed to 2.94 J / cm² ; group 3 exposed to 20.58J / cm²; and group 4 exposed to 41.16J / cm2.

The mitochondrial membrane potential (MMP) of the irradiated cellsand apoptosis assaywas assessed at 96, 120 and 144 hours.In comparison with the control populations, a significant increase in mitochondrial membrane potential (MMP) of irradiated cells was observed.

In addition, the irradiated cells showed a significant decrease in early, late apoptotic and dead cells in comparison with their respective controls.Based on the conditions of this study, we concluded that the low-level laser inhibit the apoptotic process and increase mitochondrial membrane potential (MMP).