Oocytes extraction from Iraqi local goat ovaries by using aspiration and in vitro fertilization techniques

الملخص EN

This study was conducted on 72 local slaughtered female goats, 144 ovary were collected from the different places of the city of Basrah, a local slaughterhouse, Basrah markets and agriculture station for scientific research at the university of Basrah.

Oocytes was obtaining from the ovaries by using aspiration technique and the number of it was 1044 after the division of ovarian follicular depending on the size of its diameter into two group, one < 2 mm and the second > 2.5 mm and the rate of oocytes obtained from follicles with a diameter < 2 mm (22.7 %), while the rate of oocytes obtained of follicles with a diameter > 2.5 mm (77.2 %) .these oocytes is divided depending on the number of layers of granulosa cells that surround it to: -1-Typical oocytes that contain all layers of the granulosa cells, and its rate was (10.44 %).

2 – Oocytes that invested with more than 3 layers of granulosa cells, and its rate was (48.84 %).

3 – Oocytes are partially surrounded by granulosa cells and its rate was (25.67 %).

4-denuded oocytes from granulosa cells was (15.80 %).

The highest rate of oocytes that reached to the stage of maturation were from oocytes that surrounded by more than 3 layers of granulosa cells (82.44 %).

These oocytes incubated for maturation in TCM-199 medium in 39 ᵒC, 5 % CO2 and 96% humidity for different periods (24,25,26 and 27) hours in order to determine the optimal time for maturation was obtained at the highest rate of maturity (18.29 %) within 27 hours of incubation, mostly from the follicles with diameter> 2.5 mm where the rate (19.10 %).Fertilized with sperm capacitated in vitro and the rate of fertilized oocytes (53.43 %).

Fertilized oocytes were cultured for 168 hours after fertilization at a temperature 39 ᵒC, 5% CO2 and 96% humidity to cleavage up blastocyst stage, which was represented (40 %) and stage of morula which was represented (31.42 %).