Differential effects of t helper-17 cytokines on the functions of granulocytes isolated from schistosoma mansoni-infected patients and healthy individuals
المؤلفون المشاركون
al-Shurbaji, Ayat Allah
Shata, M. T.
Muhyi, Muhammad A.
Nadi, Suad
المصدر
Journal of the Arab Society for Medical Research
العدد
المجلد 11، العدد 1 (30 يونيو/حزيران 2016)، ص ص. 29-36، 8ص.
الناشر
تاريخ النشر
2016-06-30
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background and aim The role of T helper-17 lymphocytes in the regulation of the immune response against Schistosoma soluble egg antigens is still controversial.
In this study, the invitro effects of T helper-17 cytokines [interleukin (IL)-17 and IL-22] on granulocyte functions isolated from Schistosoma-infected patients or healthy individuals were examined.
Patients and methods Twenty-seven Schistosoma mansoni-infected patients and 13 healthy individuals from Kasr Al-Aini Viral Hepatitis Center were enrolled in the present study.
Granulocytes were isolated from whole blood of patients and controls by Ficoll–Paque density gradient for removal of the mononuclear layer and then lysis of red blood cells.
Granulocytes were stimulated in vitro with soluble egg antigen in the presence of IL-17, IL-22, or both.
After 24 h, the supernatants were collected for the measurement of tumor necrosis factor (TNF)-α , hydrogen peroxide (H2O2), myeloperoxidase (MPO), and nitric oxide (NO) using enzyme-linked immunosorbent assay as surrogate markers for granulocyte functions.
Results The results indicated that the presence of IL-17 significantly decreased (P<0.05) TNF-α , H2O2, MPO, and NO production by granulocytes isolated from Schistosoma-infected patients.
In contrast, in the presence of IL-22 or both IL- 17 and IL-22, there were significant increases in the production of H2O2 and TNF-α by granulocytes isolated from Schistosoma-infected patients.
Moreover, in the presence of both IL-17 and IL-22, nonsignificant changes were observed in MPO or NO levels compared with those in the control participants.
Conclusion IL-17, in contrast to IL-22, inhibited the functional activity of granulocytes isolated from S.
mansoni-infected patients.
Therefore, neutralization of IL-17 may work as a therapeutic strategy for these patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Shurbaji, Ayat Allah& Shata, M. T.& Muhyi, Muhammad A.& Nadi, Suad. 2016. Differential effects of t helper-17 cytokines on the functions of granulocytes isolated from schistosoma mansoni-infected patients and healthy individuals. Journal of the Arab Society for Medical Research،Vol. 11, no. 1, pp.29-36.
https://search.emarefa.net/detail/BIM-696626
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Shurbaji, Ayat Allah…[et al.]. Differential effects of t helper-17 cytokines on the functions of granulocytes isolated from schistosoma mansoni-infected patients and healthy individuals. Journal of the Arab Society for Medical Research Vol. 11, no. 1 (Jan. / Jun. 2016), pp.29-36.
https://search.emarefa.net/detail/BIM-696626
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Shurbaji, Ayat Allah& Shata, M. T.& Muhyi, Muhammad A.& Nadi, Suad. Differential effects of t helper-17 cytokines on the functions of granulocytes isolated from schistosoma mansoni-infected patients and healthy individuals. Journal of the Arab Society for Medical Research. 2016. Vol. 11, no. 1, pp.29-36.
https://search.emarefa.net/detail/BIM-696626
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 35-36
رقم السجل
BIM-696626
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر