Forensic analysis of mitochondrial DNA hypervariable region HVII (encompassing nucleotide positions 37 to 340)‎ and HVIII (encompassing nucleotide positions 438-574)‎ and evaluating the importance of these variable positions for forensic genetic purposes

الملخص EN

The study aims to detection of mitochondrial hypervariable HVII and HVIII regions.

The second objective is to evaluate the importance of these positions for forensic genetic purposes and establish the degree of variation characteristic of a fragment.

Blood samples were collected from 270 healthy unrelated male living in middle and south of Iraq.

FTA® Technology is utilized to extract DNA.

A portion of a noncoding region encompassing positions 37 to 340 for HVII and encompassing positions 438 to 574 for HVIII amplified in accordance with the Anderson reference sequence.

By using EZ-10 spin column the PCR products were purified, sequenced and detected by using the ABI 3730xL Genetic Analyzer.

New polymorphic positions 57, 63 , 101, 469 and 482 are described and may in future be very important for forensic identification purpose.

This study showed the importance of the adoption of mitochondria in forensic medicine and criminal diagnosis and sites that have been discovered during the study in a privateIraqi society.

Further study on larger number of samples from different Iraqi ethic groups is suggested to confirm the results obtained by this study.