Cloning, expression, and purification of Pseudomonas aeruginosa flagellin, and characterization of the elicited anti-Flagellin antibody
المؤلفون المشاركون
Khoramabadi, Nima
Bahrudi, Mahbubih
Legaee, Parisa
Mahdawi, Mahdi
Behrouz, Bahador
Amirmozafari, Nur
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 18، العدد 6 (30 يونيو/حزيران 2016)، ص ص. 1-9، 9ص.
الناشر
تاريخ النشر
2016-06-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background : Pseudomonas aeruginosa is an important opportunistic human pathogen that causes serious infections in immunocompromised hosts.
The single polar flagellum is an important factor in both virulence and colonization.
Objectives : As flagellin is the major component of the flagellar filament, the main aims of the present study are to identify, clone, express, and purify the recombinant type B flagellin (r-B-flagellin) of P.
aeruginosa, as well as to evaluate the functional activity of the rabbit polyclonal antibody raised against this r-B-flagellin.
Materials and Methods: In the current experimental study, the r-B-flagellin gene was isolated from the P.
aeruginosa PAO1 strain by PCR.
It was cloned into the pET-28a vector and then transformed into the E.
coli BL21 strain.
Next, r-B-flagellin was overexpressed and affinity purified by Ni-NTA agarose-affinity chromatography, followed by on-column resolubilization.
Polyclonal antisera against the recombinant flagellin were raised in rabbits, and the functional activity of the anti-r-B-flagellin antibody was determined by in vitro assays.
Results : The polyclonal antibodies raised against this r-B-flagellin inhibited the motility of the homologous PAO1 strain of P.
aeruginosa, which significantly decreased the invasion of the PAO1 strain into the A549 cells and also enhanced the opsonophagocytosis of this strain.
However, our polyclonal antibody showed little effect on the heterologous PAK strain.
Conclusions : The r-B-flagellin carried antigenic epitopes just like the native flagellin, while the polyclonal antibody raised against it exhibited functional activity
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Amirmozafari, Nur& Khoramabadi, Nima& Bahrudi, Mahbubih& Legaee, Parisa& Mahdawi, Mahdi& Behrouz, Bahador. 2016. Cloning, expression, and purification of Pseudomonas aeruginosa flagellin, and characterization of the elicited anti-Flagellin antibody. Iranian Red Crescent Medical Journal،Vol. 18, no. 6, pp.1-9.
https://search.emarefa.net/detail/BIM-701546
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khoramabadi, Nima…[et al.]. Cloning, expression, and purification of Pseudomonas aeruginosa flagellin, and characterization of the elicited anti-Flagellin antibody. Iranian Red Crescent Medical Journal Vol. 18, no. 6 (Jun. 2016), pp.1-9.
https://search.emarefa.net/detail/BIM-701546
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Amirmozafari, Nur& Khoramabadi, Nima& Bahrudi, Mahbubih& Legaee, Parisa& Mahdawi, Mahdi& Behrouz, Bahador. Cloning, expression, and purification of Pseudomonas aeruginosa flagellin, and characterization of the elicited anti-Flagellin antibody. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 6, pp.1-9.
https://search.emarefa.net/detail/BIM-701546
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8-9
رقم السجل
BIM-701546
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر