Laboratory selection for resistance to anagallis arvensisas as a plant molluscicide in biomphalaria alexandrina snails

الملخص EN

The sensitivity to Anagallis arvensis as plant molluscicide was assessed in field Biomphalaria alexandrina snails and their mature progenies through successive generations by exposing them to serial concentrations of plant-powder suspension.

Survivors in 100 ppm (in parents, 1st and 2nd generations) and in 120 ppm (in 3rd, 4th and 5th generations) were allowed to produce progenies by self-fertilization for the next generation.

Also, the sensitivity of the 5th generation of B.

alexandrina which genetically selected resistant to A.

arvensis was examined to Calendula micrantha as plant molluscicide and copper sulphate and bayluscide as chemical molluscicides.

Control experiments for each variant under the same conditions using field B.

alexandrina snails were made.

Results showed that there is gradual increase in LC50 and LC90 values started from third till fifth generation followed by retardation in the 6th generation, but still higher than control.

The fifth generation which showed the maximum resistance to A.

ardencies was also more resistant to other molluscicides.

Specimens of tissue proteins from 5th generation B.

alexandrina snails (survivor in 120 ppm) and control laboratory unselected B.

alexandrina at the same shell diameter were both separated using SDS-PAGE and examined for some physiological parameters.

Dice similarity coefficient was used to compare the ecological similarity between the two snail groups using protein fractions separated by SDS-PAGE.

Results showed Dice similarity equal to 0.42 indicating that the two examined groups are different from each other in many characters.

Physiological parameters showed normal kidney functions but affected liver functions in the 5th generation snails.