استخدام تقنية Multiplex PCR في الكشف عن الجينات المنتجة للتوكسينات المعوية في بكتيريا المكورات العنقودية الذهبية المعزولة من لحوم الدجاج

الملخص EN

The aim of this study was to isolate Staphylococcus aureus from raw chicken meat and detection of enterotoxin genes in Staphylococcus aureus by using of multiplex PCR method.

Four hundred and fifty samples of raw chicken meat ( 150 samples of thighs- 150 samples of breasts -150 samples of wings ) were collected from retail market in Syria.

Bacteriological and biochemical tests results showed that out of 450 samples, 73 (17.9%) were coagulase positive staphylococcus.

Whilst the coagulase negative Staphylococcus were 334 (82.1%).

The results of multiplex PCR test conducted on S .aureus isolates, showed that 37 (50.7%) isolates harbored at least one enterotoxin gene.

The sea gene was the most frequently encountered ones.