Phylogenetic and histopathological characterization of newcastle disease viruses reveals high prevalence of very virulent strains of genotype viid in Egypt
العناوين الأخرى
التوصيف الجيني و الباثولوجي لفيروس النيوكاسل يكشف انتشار العترة شديدة الضراوة من النوع 7
المؤلفون المشاركون
Sulayman, Muhammad Ahmad
Zanati, Ali Mahmud
Hassan, Muhammad Khalifah
Hajjaj, Najla Muhammad Abd al-Aziz Husayn
Arafah, Abd al-Sattar
Said, Mahmud
المصدر
Assiut Veterinary Medical Journal
العدد
المجلد 62، العدد 150 (31 يوليو/تموز 2016)، ص ص. 168-178، 11ص.
الناشر
تاريخ النشر
2016-07-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
Newcastle disease virus (NDV) is a highly contagious disease in poultry, also considered as a major challenge for the commercial and traditional poultry industry in Egypt.
In this study clinicopathological and immunohistochemistry examination of fourteen flocks from different provinces in Egypt during 2013 and 2014 revealed velogenic ND features and positive staining for ND virus (NDV) antigen in pantropic organs of all strains which also revealed positive NDV by RRT-PCR, as it is alreadyconvinced that the fusion protein cleavage site has been deemed as responsible for the pathogenicity of NDV and selected as a target to identify and type lentogenic and velogenic strains, viral Genomic RNAs were extracted from representing swabs and organs of examined flocks, and partially amplifiedfor fusion gene of NDV using RT-PCR, then forwarded to gene sequencing.
Four chosen samples amplified for full fusion gene sequence using specific sets of newly designed primers from the available sequence data of NDV strains in Gen Bank, The resulted sequence showed motif 112RRQKRF117 which is indicative of velogenic character NDV strains, and indicate presence of 2 mutations E74D and D170N in two strains.
Phylogenetic analysis revealed that Egyptian strains are closely related to NDV genotype VIId strains of south East Asian countries based on the high nucleotide and amino acid similarity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hajjaj, Najla Muhammad Abd al-Aziz Husayn& Zanati, Ali Mahmud& Sulayman, Muhammad Ahmad& Said, Mahmud& Arafah, Abd al-Sattar& Hassan, Muhammad Khalifah. 2016. Phylogenetic and histopathological characterization of newcastle disease viruses reveals high prevalence of very virulent strains of genotype viid in Egypt. Assiut Veterinary Medical Journal،Vol. 62, no. 150, pp.168-178.
https://search.emarefa.net/detail/BIM-709066
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hajjaj, Najla Muhammad Abd al-Aziz Husayn…[et al.]. Phylogenetic and histopathological characterization of newcastle disease viruses reveals high prevalence of very virulent strains of genotype viid in Egypt. Assiut Veterinary Medical Journal Vol. 62, no. 150 (Jul. 2016), pp.168-178.
https://search.emarefa.net/detail/BIM-709066
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hajjaj, Najla Muhammad Abd al-Aziz Husayn& Zanati, Ali Mahmud& Sulayman, Muhammad Ahmad& Said, Mahmud& Arafah, Abd al-Sattar& Hassan, Muhammad Khalifah. Phylogenetic and histopathological characterization of newcastle disease viruses reveals high prevalence of very virulent strains of genotype viid in Egypt. Assiut Veterinary Medical Journal. 2016. Vol. 62, no. 150, pp.168-178.
https://search.emarefa.net/detail/BIM-709066
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 176-178
رقم السجل
BIM-709066
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر