Allele frequency of 15 autosomal short tandem repeat (STR) loci in Al Anbar : Iraqi population
المؤلفون المشاركون
al-Janabi, Dhu al-Nun Yunus
al-Zubaydi, Muhammad Mahdi
Nimah, Duha Salim
Hamid, Sura Nabil
Mahdi, Asya Abd al-Latif
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 15، العدد 2 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2016-12-31
دولة النشر
العراق
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Allele frequencies for 15 STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317,D16S539, D18S51, D21S11, FGA, TH01, TPOX, VWA, D2S1338, D19S433) included in the AmpFlSTR Identifiler kit were determined in a sample of 132 unrelated people originating From Al Anbar - Iraqi Population, samples were extracted using a Prep Filer Forensic DNA Extraction Kit.
DNA concentration measured using Nano drop Simultaneous amplifications of 15 STR loci and a gender determination marker (multiplexed PCR) were done by using the AmpFlSTR® Identifiler® PCR Amplification Kit according to the user’s manual recommendations .
The separation and detection of amplified products were conducted with the 3130 xl Genetic Analyzer 16-capillary array system following manufacturer’s protocols, A different number of alleles were observed with frequencies ranging between 0.0038 (FGA- allele 18, 19.2, 23.2, 27 and 28 - , D18S51 - allele 20 and 23, D19S433 - allele 9.2,11 and 12.2, D2S1338 – allele 27, D13S317 – allele 16, D3S1358 – allele 13 and 19 CSF1PO – allele 14, D21S11– allele 30.2 and 32 and D8S1179 - allele 9 and 17) and 0.5795 (TPOX-allele 8).
The highest heterozygosity is observed for FGA (85.60 %) where the smallest heterozygosity value is obtained for TPOX (61.36 %).
The loci were observed to have high discriminating power, as the power of discrimination of each loci varied from 0.812 (TPOX) to 0.973 (D2S1338).
All loci but D21S11 (<0.001), D19S433 (<0.001), D5S818 0.00204 and FGA (<0.00024) met Hardy- Weinberg expectations (P > 0.05).
Significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed in loci D21S11 (0.001), D19S433 (0.001), D5S818 (0.002) and FGA (0.00).
The results of the population genetics tests and pairwise comparisons suggest that these allele frequency databases are suitable for the purpose of identification in paternity or forensic investigations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Janabi, Dhu al-Nun Yunus& al-Zubaydi, Muhammad Mahdi& Nimah, Duha Salim& Hamid, Sura Nabil& Mahdi, Asya Abd al-Latif. 2016. Allele frequency of 15 autosomal short tandem repeat (STR) loci in Al Anbar : Iraqi population. Iraqi Journal of Biotechnology،Vol. 15, no. 2, pp.1-8.
https://search.emarefa.net/detail/BIM-709392
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Janabi, Dhu al-Nun Yunus…[et al.]. Allele frequency of 15 autosomal short tandem repeat (STR) loci in Al Anbar : Iraqi population. Iraqi Journal of Biotechnology Vol. 15, no. 2 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-709392
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Janabi, Dhu al-Nun Yunus& al-Zubaydi, Muhammad Mahdi& Nimah, Duha Salim& Hamid, Sura Nabil& Mahdi, Asya Abd al-Latif. Allele frequency of 15 autosomal short tandem repeat (STR) loci in Al Anbar : Iraqi population. Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 2, pp.1-8.
https://search.emarefa.net/detail/BIM-709392
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8
رقم السجل
BIM-709392
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر